The genetic diversity of microsymbionts from Thermopsis lanceolata growing in Mongolia

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Abstract

For the first time, bacteria were isolated and identified from the root nodules of a wild-growing medicinal legume plant Thermopsis lanceolata, originated from Mongolia. The taxonomic position of 14 isolates obtained was determined using of sequencing of the 16S rRNA (rrs) and atpD genes. It was shown a significant biodiversity of the isolates from T. lanceolata, which belonged to three genera of the order Rhizobiales: Phyllobacterium (family Phyllobacteriaceae), Rhizobium (family Rhizobiaceae) and Bosea (family Bradyrhizobiaceae). Six isolates belonged to the species Phyllobacterium zundukense and Phyllobacterium trifolii (100 и 99,9% rrs similarity with the type strains P. zundukense Tri-48T and P. trifolii PETP02T, respectivelly), three isolates were identified as Rhizobium anhuiense (99,8% rrs similarity with the type strain R. anhuiense CCBAU 23252T). Two slow-growing isolates of the genus Bosea Tla-534 and Tla-545 may potentially belong to new species, since their rrs-similarity to the closest type strains B. massiliensis LMG 26221T, B. lathyri LMG 26379T and B. vaviloviae Vaf18T was 98,5-99,0%. Non-rhizobial strains were not isolated. The isolation and future investigation of the rhizobial microsymbionts of the valuable medicinal legume Thermopsis lanceolata is one of the necessary prerequisites for its industrial cultivation.

About the authors

Denis S. Karlov

All-Russia Research Institute for Agricultural Microbiology

Author for correspondence.
Email: makondo07@gmail.com
SPIN-code: 8355-8091
ResearcherId: E-2552-2014

PhD, Junior Researcher, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Anna L. Sazanova

All-Russia Research Institute for Agricultural Microbiology

Email: anna_sazanova@mail.ru

PhD, Senior Researcher, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Irina G. Kuznetsova

All-Russia Research Institute for Agricultural Microbiology

Email: kuznetsova_rina@mail.ru

Engineer-Researcher, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Vera I. Safronova

All-Russia Research Institute for Agricultural Microbiology

Email: v.safronova@rambler.ru

PhD, Head, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Nina Y. Tikhomirova

All-Russia Research Institute for Agricultural Microbiology

Email: arriam2008@yandex.ru

Researcher, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Zhanna P. Popova

All-Russia Research Institute for Agricultural Microbiology

Email: elestd@yandex.ru

PhD, Senior Researcher, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Yuriy S. Osledkin

All-Russia Research Institute for Agricultural Microbiology

Email: arriam2008@yandex.ru

PhD, Leading Researcher, Russian Collection of Agricultural Microorganisms

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

Alla V. Verkhozina

Siberian Institute of Plant Physiology and Biochemistry (SIPPB)

Email: allaverh@list.ru

PhD, Head of The Herbarium Group, Department of Terrestrial Ecosystems Resistance

Russian Federation, 132, Lermontova street, Irkutsk, 664033

Andrey A. Belimov

All-Russia Research Institute for Agricultural Microbiology

Email: belimov@rambler.ru

DrSci, Head of Laboratory of Rhizosphere Microflora

Russian Federation, 3, Podbelsky highway, Pushkin, Saint-Petersburg, 196608

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2. Fig. 1. Phylogenetic tree generated by the neighbour-joining method using partial 16S rRNA gene sequences of the isolated strains from Thermopsis lanceolata nodules and representatives of closely related to Phyllobacterium and Rhizobium species. The isolated strains in bold. Type species are indicated by the letter T. I–III clusters that formed by Phyllobacterium isolates obtained in the work. Bootstrap values more than 50% are given

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3. Fig. 2. Phylogenetic tree generated by the neighbour-joining method using atpD gene sequences of the isolated strains from Thermopsis lanceolata nodules and representatives of closely related to Phyllobacterium and Rhizobium species. The isolated strains in bold. Type species are indicated by the letter T. Iа, Ib, II и III – clusters that formed by Phyllobacterium isolates obtained in the work. Bootstrap values more than 50% are given

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4. Fig. 3. Phylogenetic tree generated by the neighbour-joining method using partial 16S rRNA gene sequences of the isolated strains from Thermopsis lanceolata nodules and representatives of closely related to Bosea species. The isolated strains in bold. Type species are indicated by the letter T. Bootstrap values more than 50% are given

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5. Fig. 4. Phylogenetic tree generated by the neighbour-joining method using atpD gene sequences of the isolated strains from Thermopsis lanceolata nodules and representatives of closely related to Bosea species. The isolated strains in bold. Type species are indicated by the letter T. Bootstrap values more than 30% are given

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Copyright (c) 2019 Karlov D.S., Sazanova A.L., Kuznetsova I.G., Safronova V.I., Tikhomirova N.Y., Popova Z.P., Osledkin Y.S., Verkhozina A.V., Belimov A.A.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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