Specificity of the symbiotic interaction of bacteria of the genus Rhizobium leguminosarum bv. viciae with plants of the tribe Vicieae

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Abstract

The estimation of nodulation competitiveness of industrial strains against the native nodule bacteria and also the analyses of distribution of strain’s genotypes which formed nodules on roots of 4 plant species was the purpose of this work. The objects of the research were rhizobium strains which formed nodules on roots of plant (the nodule-forming units – NFU), obtained in field experiment with application of preseeding processing of seeds of pea (Pisum satіvum L.), fava beans (Vicia faba L.), lathyrus (Lathyrus sativus L.) and lentil (Lens culinaris L.). The mixture of the collection strains allocated from nodules of peas and beans, and having various combinations of chromosomal and symbiotic genotypes was used for inoculation of seeds. Identification of NFU was carried out with the use of the analysis of the emitted nodule total DNA on several chromosomal markers: the rpoB gene and the hin-region, and the plasmid marker – nodD gene. It is established that only about 50% of nodules were formed by the strains used at inoculation of seeds. Besides, the combinations of chromosomal and symbiotic genotypes specific for a rhizobium – symbionts of concrete host plants have been established: IA genotype with sym-2 for P. sativum; IB genotype with sym-4 for V. faba. The results of this study create prerequisites for selection of couples: macro- and microsymbiont for the purpose of increasing efficiency of plant-microbial systems, in which the nature of symbiotic interaction defines efficiency of partners.

About the authors

Sofya A. Khapchaeva

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Author for correspondence.
Email: sakhapchaeva.1990@gmail.com
ORCID iD: 0000-0002-6900-8399
SPIN-code: 2456-8389
ResearcherId: H-9438-2014

Junior Researcher of Algal Biotechnology Group

Russian Federation, 33, bld. 2 Leninsky Ave., Moscow 119071

Svetlana V. Didovich

Research Institute of Agriculture of Crimea

Email: sv-alex.68@mail.ru
SPIN-code: 4162-1908

Ph. D, Agriculture, Senior Research Scientist, Agricultural Microbiology Department

Russian Federation, 150 Kievskaya str., Simferopol, 295493

Alexey F. Topunov

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: aftopunov@yandex.ru
SPIN-code: 8379-6221

Dr. Sci. (Biology), Сhief Research Scientist, Head of the Laboratory of Biochemistry of Nitrogen Fixation and Nitrogen Metabolism

Russian Federation, 33, bld. 2 Leninsky Ave., Moscow

Andrey L. Mulyukin

S.N. Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences

Email: andlm@mail.ru
SPIN-code: 1269-5440

Dr. Sci. (Biology), Leading Researcher, Head of the UNIQEM Collection Core Facility

Russian Federation, 33, bld. 2 Leninsky Ave., Moscow 119071

Vasiliy S. Zotov

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences

Email: adni83@yandex.ru

Ph. D, Biology, Senior Research Scientist, Head of the Group of Algae Biotechnology

Russian Federation, 33, bld. 2 Leninsky Ave., Moscow 119071

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Supplementary files

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2. Fig. 1. The phylogenetic tree that was constructed on the basis of comparative sequence analysis of the fragment of rpoB gene of Rhizobium sp. – symbionts of cross-inoculation group plants using the NJ algorithm. Scale corresponds to 2 replacements by 100 couples of the bases. The numerals show the statistical reliability of the order of branching (%) defined by bootstrap analysis (1000 replicas). Bootstrap values less than 70% are not shown

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3. Fig. 2. The RFLP analysis of a fragment of nodD gene with use of a MspI restriction endonuclease. 50+ bp DNA Ladder. Electrophoretic profile for 12 nodule-forming units of each species of a plant are presented: lentil, lathyrus, fava beans and pea. Sym-genotypes are designated by figures (1-4)

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4. Fig. 3. Diagram of distribution of sym-genotypes in the nodule-forming units of cross-inoculation group plants: lentil, lathyrus, fava beans and pea

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5. Fig. 4. Diagram of distribution of hin-genotypes in the nodule-forming units of cross-inoculation group plants: lentil, lathyrus, fava beans and pea

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6. Fig. 5. Structure of the hin-region of Rhizobium leguminosarum bv. viciae

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Copyright (c) 2018 Khapchaeva S.A., Didovich S.V., Topunov A.F., Mulyukin A.L., Zotov V.S.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
 


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