Morphological features of brain damage in severe COVID-19
- Authors: Kanibolotskiy A.A.1,2, Zayratyants O.V.3
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Affiliations:
- N.V. Sklifosovsky Research Institute for Emergency Medicine
- Research Institute for Healthcare Organization and Medical Management
- Moscow State University of Medicine and Dentistry named after A.I. Evdokimov
- Issue: Vol 14, No 1 (2023)
- Pages: 21-30
- Section: Original Study Articles
- URL: https://journals.rcsi.science/clinpractice/article/view/142799
- DOI: https://doi.org/10.17816/clinpract176827
- ID: 142799
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Abstract
Background: The damage to the nervous system in COVID-19 reflects the systemic nature of the infection. The question of the neuroinvasive potential of SARS-CoV-2 remains open, the role of "pseudovirions" in the development of the endothelial dysfunction, as well as of the S1 subunit in the TLR activation, and the importance of the blood-brain barrier are discussed. The immunological, non-immunological, and cytopathic mechanisms of the virus's action are described; there is no clear understanding of the genesis of neuropathological changes caused by SARS-CoV-2. In this tragic pandemic, the lessons of the dead should help save lives and health.
Aim: to study and explain the features of brain damage in COVID-19.
Methods: Brain fragments from 20 patients who died due to severe COVID-19 were studied, the sections were stained with hematoxylin and eosin, according to van Gieson and Nissl, IHC reactions were performed with antibodies to the S-protein, CD68 and CD8, the changes were compared with those related to the lethal outcomes of pancreatic necrosis and ruptured aortic aneurysm.
Results: The following changes in the olfactory analyzer were revealed: sharp edema, dystrophic changes in neurons, gliosis, accumulations of starchy bodies, which explains the neuronal pathway of SARS-CoV-2 invasion; vascular plethora, erythrostasis and thrombosis, perivenular hemorrhages, diffuse edema, macroglia proliferation, perivascular astrocytosis and satellite. A positive reaction with the antibodies to the S1 and S2 subunits of the spike protein was detected, while the result of the reaction with antibodies to the N-protein of the virus, confirming the active replication of the virus, was doubtful. The S-protein expression in individual endotheliocytes makes the transendothelial route of the virus entry unlikely, in contrast to the hematogenous and neuronal pathways. The viral DNA was not detected by PCR. A weak inflammatory reaction was revealed in the form of perivascular accumulations of lymphocytes, scattered T-lymphocytes.
Conclusions: 2 groups of changes were identified, the first group included circulatory disorders with a tendency to thrombosis, edema, dystrophic-necrotic changes in neurons, glial proliferation, the second group included inflammatory-degenerative changes, a weak inflammatory reaction and amyloid-like bodies. Further morphometric and statistical studies are needed to obtain the reliable conclusions.
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##article.viewOnOriginalSite##About the authors
Aleksander A. Kanibolotskiy
N.V. Sklifosovsky Research Institute for Emergency Medicine; Research Institute for Healthcare Organization and Medical Management
Author for correspondence.
Email: dr.kaa@mail.ru
SPIN-code: 3976-1662
врач-патологоанатом патологоанатомического отделения, заведующий организационно-методическим отделом по патологической анатомии
Russian Federation, Moscow; MoscowOleg V. Zayratyants
Moscow State University of Medicine and Dentistry named after A.I. Evdokimov
Email: ovzair@mail.ru
ORCID iD: 0000-0003-3606-3823
SPIN-code: 4817-1084
MD, PhD, Professor
Russian Federation, MoscowReferences
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