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Post a Comment Sensitivity of antibiotic resistant coagulase-negative staphylococci to antiseptic piсloxydin
- Authors: Khalatyan A.S.1, Budzinskaya M.V.1, Kholina E.G.2, Strakhovskaya M.G.2,3, Kolyshkina N.A.4, Kovalenko I.B.2,3, Zhukhovitsky V.G.5
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Affiliations:
- Research Institute of Eye Diseases
- Lomonosov Moscow State University
- Federal Scientific and Clinical Center of Specialized Types of Medical Care and Medical Technologies of the Federal Medical and Biological Agency of Russia
- Federal Scientific and Clinical Center of Specialized Types of Medical = Care and Medical Technologies of the Federal Medical and Biological Agency of Russia
- N.F. Gamaleya National Research Center of Epidemiology and Microbiology
- Issue: Vol 11, No 1 (2020)
- Pages: 42-48
- Section: Basic Science
- URL: https://journals.rcsi.science/clinpractice/article/view/17543
- DOI: https://doi.org/10.17816/clinpract17543
- ID: 17543
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Abstract
Background. Coagulase-negative staphylococci (CNS), primarily Staphylococcus epidermidis, predominate in the normal microflora of the eye. However, due to irrational antibiotic therapy, resistant strains are widely distributed among CNS.
Aim. To study the sensitivity of the antibiotic resistant CNS isolates to picloxydine, an antiseptic.
Methods. The species, sensitivity to antibiotics and picloxydine were determined for 39 isolates of bacteria obtained from the conjunctival swabs. The cells’ morphology under the antiseptic’s influence was studied by electron microscopy.
Results. 33 isolates of S. epidermidis (17 sensitive or resistant to drugs of no more than 2 classes of antibiotics and 16 MDR), 2 S. haemolyticus (1 resistant to 2 classes of antibiotics and 1 MDR), 3 S. hominis (1 sensitive and 2 MDR), 1 S. caprae (MDR) were characterized. In in vitro tests, picloxydine showed high efficiency in suppressing the growth of staphylococci regardless of their sensitivity to antibiotics, as well as bactericidal activity at concentrations of 15.6–31.2 µg/ml, close to those of chlorhexidine. At these concentrations, the antiseptic had a destructive effect on the surface structures of bacterial cells.
Conclusion. The picloxydine antiseptic is equally effective against antibiotic- sensitive and antibiotic-resistant coagulase-negative staphylococci.
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##article.viewOnOriginalSite##About the authors
A. S. Khalatyan
Research Institute of Eye Diseases
Email: anaits92@gmail.com
ORCID iD: 0000-0001-6255-5544
аспирант, младший научный сотрудник
Russian Federation, MoscowM. V. Budzinskaya
Research Institute of Eye Diseases
Email: m_budzinskaya@mail.ru
ORCID iD: 0000-0002-5507-8775
SPIN-code: 3552-7061
д.м.н., ведущий научный сотрудник, заместитель директора по научной работе, руководитель отдела патологии сетчатки и зрительного нерва
Russian Federation, MoscowE. G. Kholina
Lomonosov Moscow State University
Email: tenarra@mail.ru
ORCID iD: 0000-0002-5918-5084
аспирант биологического факультета
Russian Federation, MoscowM. G. Strakhovskaya
Lomonosov Moscow State University; Federal Scientific and Clinical Center of Specialized Types of Medical Care and Medical Technologies of the Federal Medical and Biological Agency of Russia
Author for correspondence.
Email: maristra@yandex.ru
ORCID iD: 0000-0002-4571-0431
SPIN-code: 6343-1297
Scopus Author ID: 6603888541
ResearcherId: I-5529-2012
д.б.н., ведущий научный сотрудник биологического факультета
Russian Federation, MoscowN. A. Kolyshkina
Federal Scientific and Clinical Center of Specialized Types of Medical = Care and Medical Technologies of the Federal Medical and Biological Agency of Russia
Email: baklab_83@mail.ru
заведующая клинико-диагностической лабораторией
Russian Federation, MoscowI. B. Kovalenko
Lomonosov Moscow State University; Federal Scientific and Clinical Center of Specialized Types of Medical Care and Medical Technologies of the Federal Medical and Biological Agency of Russia
Email: ikovakenko78@gmail.com
ORCID iD: 0000-0002-4949-6591
д.физ.-мат.н., ведущий научный сотрудник биологического факультета
Russian Federation, MoscowV. G. Zhukhovitsky
N.F. Gamaleya National Research Center of Epidemiology and Microbiology
Email: zhukhovitsky@rambler.ru
к.м.н., руководитель лаборатории индикации и ультраструктурного анализа микроорганизмов
Russian Federation, MoscowReferences
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