ANTI-FUNGAL ACTIVITY OF ACTINOMYCETES ALLOCATED FROM THE SURFACE OF ROOTS OF MEDICINAL PLANTS

The need for the use of antifungal drugs has increased significantly due to an increase in the prevalence of mycoses, due to the increased number of patients with immunosuppression. The introduction of new antifungal drugs, and, therefore, a preliminary study of the topic, expands the possibilities of treating fungal infections. Actinomycetes are the producers of most antimicrobials, among the drugs available on the market. Objective: to determine the antagonistic relationship of actinomycetes isolated from the surface of the roots of medicinal plants with pathogenic fungi of the mycelial and non-mycelial structure. The tasks were set: 1) to study the antagonistic activity of actinomycetes in relation to pathogenic fungi; 2) to identify active strains of actinomycetes for further study of the composition and mechanisms of action of their metabolites; 3) determine the best method for studying antagonistic activity; 4) to consider the stability of biologically active substances synthesized by actinomycetes in the presence of positive antagonistic relationships. The study of antifungal activity against yeast fungi was carried out by 2 methods: “perpendicular lines” and “blocks”, in order to identify the best. In a parallel study using the “block” method, we studied the duration of the activity of actinomycete metabolites, for which we measured the diameter of the growth suppression zone around the blocks for 1 week. The activity of actinomycetes against mycelial fungi was determined by the block method. All studies were performed in duplicate. Results: 1) of the 16 studied strains of actinomycetes, antifungal activity in at least one test culture was detected in 9 isolates, which is about 56%; 2) the best method of study was the "block" method, which allowed to identify 50% of the antagonists among the studied strains, while the "line" method, which is only 19%; 3) the most active actinomycromycetic isolate was the strain Streptomyces sp. with an index of 3-PP.

Actinomycetales, Candida, Aspergillus, Actinomycetales, Candida, Aspergillus, mycosis, candidiasis, antagonistic activity, pathogenic fungi, radiant mushrooms, “block” method, “line” method