INFLUENCE FEATURES OF EXOMETABOLITES OF CAT TAPEWORM CYSTICERCUS ON THE PROLIFERATIVE ACTIVITY OF L929 CELLS

Tissue parasites in the host organism cause a number of local and general changes aimed at ensuring a long-term existence. They secrete a complex of biologically active substances that stimulate the proliferation of various cells. This work is a fragment of a comprehensive study of relationships in host-parasite systems in tissue larval helminthiases. The purpose of this study was to investigate the influence of the nature of the cat tapeworm cysticerci exometabolites on some parameters of the proliferative activity of L929 fibroblast line. Fibroblasts were cultured on media containing cysticercal metabolites. The level of proliferative activity of fibroblasts was assessed by the number of mitoses and cloning efficiency. It was shown that exometabolites of feline cysticerci have wide spectra of biological action. The variety of effects caused by exometabolites probably allows the parasite to flexibly adapt to the conditions of parasitization in host tissues, act on various links of its homeostasis and maintain a certain level of specialization and functional activity of various target cells. Exometabolites of feline chain cysticerci alter the level of proliferative activity, the degree and direction of differentiation of connective tissue and epithelial cells in cultures. The nature of this effect is determined by the belonging of cells to one or another tissue type, the degree of their differentiation, as well as the dose of exometabolites and, probably, the ratio of their various components. The dependence of the nature of the influence of exometabolites of cysticerci on the proliferation of L929 cells on the presence of serum in the culture medium was studied. The cultivation of L929 fibroblasts on the medium with the greatest inhibitory effect was accompanied by a characteristic fatty degeneration of these cells, which can be considered as stimulation of differentiation of L929 fibroblasts along the pathway of conversion into adipocytes.

Parasitoses, tissue helminthiases, cysticerci, proliferation, fibroblasts, cell cultivation, line L929, cloning efficiency, mitosis