Mitochondrial networks of cumulus cells and oocyte quality
- 作者: Panferov E.V.1, Tapilskaya N.I.2, Masieva K.S.3, Ob’edkova K.V.2, Gzgzyan A.M.2
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隶属关系:
- Institute of Cytology Russian Academy of Science
- Ott Research Institute of Obstetrics, Gynecology and Reproductology
- Military Medical Academy
- 期: 卷 41, 编号 3 (2022)
- 页面: 303-308
- 栏目: Original articles
- URL: https://journals.rcsi.science/RMMArep/article/view/109074
- DOI: https://doi.org/10.17816/rmmar109074
- ID: 109074
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BACKGROUND: Mitochondria play vital roles in oocyte functions and they are critical indicators of oocyte quality which is important for fertilization and development into viable offspring. Quality-compromised oocytes in which mitochondrial dysfunction plays a significant role are correlated with infertility, developmental disorders and embryo loss. A significant role in the oocytes of cytoplasmic accumulation and a sufficient amount of the mitochondrial pool of oocytes is played by the cumulus cells surrounding it.
AIM: to conduct a comparative analysis between the state of mitochondrial networks of cumulus cells and the quality of oocytes.
MATERIALS AND METHODS: The study included 22 women aged 28 to 37 years inclusive (mean age 32.3 ± 1.2 years) with tubal infertility. During the assisted reproductive technology procedures, 74 oocytes were obtained. 39 good quality oocytes and their cumulus cells were compared with 35 bad quality oocytes and their cumulus cells respectively. After puncture of ovarian follicles cumulus cells were isolated and stained with fluorescent dye for cell tracing and mitochondrial imaging in vivo. The method of confocal microscopy was used to analyze the three-dimensional organization of mitochondria in 20–30 cells of one pool of follicular fluid. The following parameters were used to evaluate the mitochondrial network: fluorescence decay time and fluorescence intensity.
RESULTS: The decay time of fluorescence in cumulus cells associated with a good quality oocyte was significantly higher (p = 0.032) than in the case of identification of oocytes with negative signs.
CONCLUSION: The structure of mitochondrial networks in cumulus cells correlates (r = 0.76) with the quality of oocytes.
作者简介
Egor Panferov
Institute of Cytology Russian Academy of Science
Email: panferov.aux@gmail.com
master of the Institute of cytology of the Russian Academy of Sciences
俄罗斯联邦, Saint PetersburgNatalya Tapilskaya
Ott Research Institute of Obstetrics, Gynecology and Reproductology
编辑信件的主要联系方式.
Email: tapnatalia@yandex.ru
ORCID iD: 0000-0001-5309-0087
SPIN 代码: 3605-0413
Scopus 作者 ID: 23013489000
Researcher ID: A-7504-2016
M.D., D.Sc. (Medicine), Professor, leading research scientist
俄罗斯联邦, Saint PetersburgKristina Masieva
Military Medical Academy
Email: masieva98@mail.ru
6th year student
俄罗斯联邦, Saint PetersburgKseniya Ob’edkova
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: obedkova_ks@mail.ru
ORCID iD: 0000-0002-2056-7907
SPIN 代码: 2709-2890
M.D., Ph.D. (Medicine), research scientist of the Reproduction Department
俄罗斯联邦, Saint PetersburgAlexander Gzgzyan
Ott Research Institute of Obstetrics, Gynecology and Reproductology
Email: iagmail@ott.ru
ORCID iD: 0000-0003-3917-9493
SPIN 代码: 6412-4801
Scopus 作者 ID: 56232643300
M.D., D.Sc. (Medicine), Head of the Reproduction Department
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