Reactive changes of gastric mucosa and reduction of desacyl grelin in rat brain due to psychoemotional stress
- Authors: Raptanova V.A.1,2, Droblenkov A.V.1,2, Lebedev A.A.1, Bobkov P.S.1,2, Khokhlov P.P.1, Thyssen I.Y.1, Lisovskiy A.D.1, Bychkov E.R.1, Shabanov P.D.1
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Affiliations:
- Institute of Experimental Medicine
- St. Petersburg Medicо-Social Institute
- Issue: Vol 19, No 2 (2021)
- Pages: 203-210
- Section: Original study articles
- URL: https://journals.rcsi.science/RCF/article/view/77313
- DOI: https://doi.org/10.17816/RCF192203-210
- ID: 77313
Cite item
Abstract
BACKGROUND: The work is devoted to the analysis of the elements the reactivity of grelin system in the model of psychogenic stress. In recent years, it has been shown that the ghrelin brain system is not limited only to the regulation of energy balance and eating behavior. Along with other peptide regulatory systems, it plays an important role in the mechanisms of stress, reward and addiction. Therefore, the elements of this system should be considered primarily as molecular targets of pharmacological action in order to correct the states of addiction and post-stress disorders.
MATERIALS AND METHODS: To produce psychoemotional stress, we used an acute single traumatic situation in male Wistar rats. The animals were placed in the tiger python, one animal died as a result of its nutritional needs, the rest of the rats experienced the death of a partner. One week after exposure to python, the animals were decapitated, and the brain structures were isolated. Aliquots of the brain structures suspensions were examined for the content of desacyl ghrelin (DAG) using a highly sensitive enzyme-linked immunosorbent assay (ELISA). In another group, rats were decapitated on the 4th day after exposure to python, stomachs were removed, which were fixed in 10% formalin solution. In horizontal paraffin sections of the gastric mucosa, after staining with hematoxylin and eosin, the heights of superficial and dimple mucous cells, the height of the dimple stroma, the area of superficial, dimple mucocytes and stroma of the dimples, and the number of dead mucocytes were calculated. To clarify the differentiation of epithelial cells, they were stained with alcian blue
RESULTS: DAG was detected in all studied brain structures: amygdala, hippocampus, and hypothalamus. The highest concentration of DAG was noted in the hypothalamus (p < 0.05), which may serve as an indirect confirmation of the data on the presence of ghrelin-containing neurons in the nuclei of the hypothalamus. After exposure to stress, a sharp decrease in the level of DAG was observed in all studied brain structures (8–12 times, p < 0.01): amygdala, hippocampus, and hypothalamus. It has been established that the experience of the stress of the death of a partner is expressed by erosive inflammation of the gastric mucosa, the death of many mucous cells, and an increase in mucus production in viable epithelial cells.
CONCLUSION: Psychoemotional stress completely suppresses the content of desacyl ghrelin of the brain in rats, which may be based on both a disturbance of the central mechanisms of limbic regulation and a violation of peripheral mechanisms, in particular, reactive changes in the gastric mucosa.
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##article.viewOnOriginalSite##About the authors
Vladislava A. Raptanova
Institute of Experimental Medicine; St. Petersburg Medicо-Social Institute
Author for correspondence.
Email: vladislavaraptanova@yandex.ru
postgraduate student
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376; Saint PetersburgAndrei V. Droblenkov
Institute of Experimental Medicine; St. Petersburg Medicо-Social Institute
Email: droblenkov_a@mail.ru
ORCID iD: 0000-0001-5155-1484
SPIN-code: 8929-8601
PhD, Dr. Sci. (Med.)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376; Saint PetersburgAndrei A. Lebedev
Institute of Experimental Medicine
Email: aalebedev-iem@rambler.ru
ORCID iD: 0000-0003-0297-0425
SPIN-code: 4998-5204
PhD, Dr. Sci. (Biol.), Professor
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376Pavel S. Bobkov
Institute of Experimental Medicine; St. Petersburg Medicо-Social Institute
Email: bobkov_pl@mail.ru
ORCID iD: 0000-0003-4858-6170
SPIN-code: 2382-8246
PhD, Cand. Sci. (Med.)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376; Saint PetersburgPlaton P. Khokhlov
Institute of Experimental Medicine
Email: platonkh@list.ru
ORCID iD: 0000-0001-6553-9267
SPIN-code: 8673-7417
PhD, Cand. Sci. (Biochemistry)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376Ilia Y. Thyssen
Institute of Experimental Medicine
Email: iljatis@mail.ru
ORCID iD: 0000-0002-8710-9580
SPIN-code: 9971-3496
PhD, Cand. Sci. (Biol.)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376Anatoliy D. Lisovskiy
Institute of Experimental Medicine
Email: lisovskiy.t@mail.ru
postgraduate
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376Eugeny R. Bychkov
Institute of Experimental Medicine
Email: bychkov@mail.ru
PhD, Cand. Sci. (Med.)
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376Petr D. Shabanov
Institute of Experimental Medicine
Email: pdshabanov@mail.ru
ORCID iD: 0000-0003-1464-1127
SPIN-code: 8974-7477
MD, PhD, Dr. Sci. (Med.), Professor
Russian Federation, 12, Acad. Pavlov str., Saint Petersburg, 197376References
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