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Post a Comment Conditioned place preference of kisspeptin-10
- Authors: Tissen I.Y.1, Chepik P.A.1, Lebedev A.A.1, Magarramova L.A.1, Bychkov E.R.1, Shabanov P.D.1
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Affiliations:
- Institute of Experimental Medicine
- Issue: Vol 19, No 1 (2021)
- Pages: 47-53
- Section: Original study articles
- URL: https://journals.rcsi.science/RCF/article/view/70617
- DOI: https://doi.org/10.17816/RCF19147-53
- ID: 70617
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Abstract
INTRODUCTION: Kisspeptins (KISS), a group of brain neuropeptides are involved in sexual behavior. KISS activate the hypothalamic neurons that synthesize gonadotropin releasing hormone. KISS was also detected in the limbic system. Earlier, we showed the activation of sexual motivation after the administration of kisspeptin-10 without increasing the level of testosterone in male rats, which suggests the extrahypothalamic effect of KISS.
The aim of this work was to study the possibility of aquisition of conditioned place preference of kisspeptin-10, as well as to study the emotional and investigational behavior in rats after intranasal peptide administration.
METHODS: Conditioned place preference test (CPP), “open field” test (OP) and “elevated plus maze” (EPM) were used in male Wistar rats.
RESULTS: When studying CPP, animals spent 78.6 ± 6.3% of the time in the chamber associated with the administration of KISS compared to control animals with administration of physiological saline (51.2% of the experiment time; p < 0.05). After kisspeptin-10 administration locomotor activity was 2-fold increased (p < 0.05), and the number of sniffings was 2-fold increased too (p < 0.05). The parameters did not significantly differ in animals treated with kisspeptin or saline in PCL.
CONCLUSION: Thus repeated intranasal administration of kisspeptin-10 induces the aquisition of CPP in rats. This suggests that kisspeptin-10 can cause activity in the reward system or the activation of brain regions associated with this system, which ultimately leads to the formation of an emotionally positive state.
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##article.viewOnOriginalSite##About the authors
Ilia Yu. Tissen
Institute of Experimental Medicine
Author for correspondence.
Email: iljatis@mail.ru
ORCID iD: 0000-0002-8710-9580
SPIN-code: 9971-3496
PhD, Cand. Sci. (Biol.), Senior Researcher
Russian Federation, 12 Acad. Pavlov str., Saint Petersburg, 197376Polina A. Chepik
Institute of Experimental Medicine
Email: aalebedev-iem@rambler.ru
Post-graduate Fellow
Russian Federation, 12 Acad. Pavlov str., Saint Petersburg, 197376Andrei A. Lebedev
Institute of Experimental Medicine
Email: aalebedev-iem@rambler.ru
ORCID iD: 0000-0003-0297-0425
SPIN-code: 4998-5204
Dr. Biol. Sci. (Pharmacology)
Russian Federation, 12 Acad. Pavlov str., Saint Petersburg, 197376Leila A. Magarramova
Institute of Experimental Medicine
Email: alexandrablazhenko@gmail.com
Post-graduate
Russian Federation, 12 Acad. Pavlov str., Saint Petersburg, 197376Eugenii R. Bychkov
Institute of Experimental Medicine
Email: bychkov@mail.ru
ORCID iD: 0000-0002-8911-6805
SPIN-code: 9408-0799
PhD (Pathophysiology), Cand. Sci. (Med.)
Russian Federation, 12 Acad. Pavlov str., Saint Petersburg, 197376Petr D. Shabanov
Institute of Experimental Medicine
Email: pdshabanov@mail.ru
ORCID iD: 0000-0003-1464-1127
SPIN-code: 8974-7477
Dr. Med. Sci. (Pharmacology), Professor
Russian Federation, 12 Acad. Pavlov str., Saint Petersburg, 197376References
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