Email this article 
Post a Comment Comparative effectiveness of Molixan and Glutoxim in the model of toxic liver lesions with anti-tuberculosis drugs
- Authors: Bugaev P.A.1, Basharin V.A.1, Antushevich A.E.1
-
Affiliations:
- S.M. Kirov Military Medical Academy
- Issue: Vol 17, No 2 (2019)
- Pages: 49-54
- Section: Original study articles
- URL: https://journals.rcsi.science/RCF/article/view/15616
- DOI: https://doi.org/10.17816/RCF17249-54
- ID: 15616
Cite item
Full Text
Abstract
Due to the high frequency of liver lesions caused by anti-tuberculosis drugs, the possibility of using compounds based on oxidized glutathione for hepatoprotection is shown.
Purposes and tasks. The purpose of the study was to conduct a comparative assessment of the hepatoprotective properties of molixan and glutoxim in liver lesions with anti-tuberculosis drugs. To achieve this goal, it was necessary to perform the following tasks: on the model of toxic liver damage caused by anti-tuberculosis drugs (isoniazid, rifampicin, pyrazinamide) to study the effectiveness of glutoxin and molixan by biochemical and morphological parameters; on the basis of the data to determine the most effective scheme of application.
Materials and methods: the study was conducted on 90 white male not inbred rats. Antituberculosis drugs (AD) were administered to animals daily for 14 days in the following doses: isoniazid – 50 mg/kg intraperitoneal, rifampicin – 250 mg/kg and pyrazinamide – 45 mg/kg intragastrically. Glutoxim and molixan were administered daily for 14 days at doses: 20 and 40 mg/kg – glutoxim, 30 mg/kg – molixan, intraperitoneal for 2 hours before the introduction of anti-tuberculosis drugs. The animals were divided into 5 groups of 18 rats each. Group 1 – intact animals, 2 – control AD, 3 – AD + glutoxim 20 mg/kg, 4 – AD + glutoxim 40 mg/kg, 5 – AD + molixan 30 mg/kg. In the study on day 15, biochemical parameters of blood were determined, the liver was taken for morphohistological study and the level of LPO in its homogenates was determined by the concentration of diene conjugates and malondialdehyde.
Results and conclusions. As a result of the experimental study, it was noted that the more pronounced hepatoprotective effect was exerted by molixan.
Full Text
##article.viewOnOriginalSite##About the authors
Pjotr A. Bugaev
S.M. Kirov Military Medical Academy
Author for correspondence.
Email: petr-bugaev@mail.ru
Head of Department of Clinic Military-field Therapy
Russian Federation, 6G, Akademika Lebedeva street, Saint-Petersburg, 194044Vadim A. Basharin
S.M. Kirov Military Medical Academy
Email: basharin1@mail.ru
Doctor of Medical Sciences, Professor, Head of the Department of Military Toxicology and Medical Protection
Russian Federation, 6G, Akademika Lebedeva street, Saint-Petersburg, 194044Aleksandr E. Antushevich
S.M. Kirov Military Medical Academy
Email: a.antushevich@mail.ru
Doctor of Medical Sciences, Senior Researcher of the Research Laboratory of Military Therapy of the Research Center
Russian Federation, 6G, Akademika Lebedeva street, Saint-Petersburg, 194044References
- Антушевич А.Е., Бузанов Д.В., Антонов В.Г., и др. Оценка эффективности применения инозина глицил-цистеинил-глутамата динатрия при острых тяжелых отравлениях этанолом // Вестник Российской военно-медицинской академии. – 2017. – № 2. – С. 7–12. [Antushevich AE, Buzanov DV, Antonov VG, et al. Evaluation of effectiveness of inosine glycyl-cysteinyl-glutamate disodium in acute severe poisoning with ethanol. Vestnik Rossiiskoi voenno-medicinskoi akademii. 2017;(2):7-12. (In Russ.)]
- Васильева С.Н. Экспериментальное обоснование использования глутоксима в качестве средства сопровождения этиотропной терапии генерализованного туберкулеза: Автореф. дис. … канд. мед. наук. – СПб., 2004. – 24 с. [Vasil’eva SN. Ehksperimental’noe obosnovanie ispol’zovaniya glutoksima v kachestve sredstva soprovozhdeniya ehtiotropnoi terapii generalizovannogo tuberkuleza. [dissertation abstract] Saint Petersburg; 2004. 24 р. (In Russ.)].Доступно по: https://search.rsl.ru/ru/record/01003242496. Ссылка активна на 16.12.2018.
- Гребенюк А.Н., Рейнюк В.Л., Антушевич А.Е., и др. Эффективность гепатопротектора с пептидным компонентом моликсана при острой крайне тяжелой интоксикации этанолом // Токсикологический вестник. – 2014. – № 4. – С. 18–24. [Grebenyuk AN, Reiniuk VL, Antushevich AE, et al. Effectiveness of hepatoprotector with peptide component molixan in treatment of heaviest acute intoxications by. Toxicological Review. 2014;(4):18-24. (In Russ.)]
- Иванова Д.А. Нежелательные побочные реакции при лечении больных туберкулезом // Туберкулез и болезни легких. – 2011. – № 36. – С. 60–69. [Ivanova DA. Nezhelatel’nye pobochnye reakcii pri lechenii bol’nyh tuberkulyozom. Tuberculosis and lung diseases. 2011;(36):60-69. (In Russ.)]
- Наумова А.А. Влияние глутоксима и моликсана на внутриклеточную концентрацию Са2+ в макрофагах: роль каскада метаболизма арахидоновой кислоты и актинового цитоскелета: Автореф. дис. … канд. биол. наук. – СПб., 2017. – 23 с. [Naumova AA. Vliyanie glutoksima i moliksana na vnutrikletochnuyu koncentraciyu Ca2+ v makrofagah: rol’ kaskada metabolizma arahidonovoi kisloty i aktinovogo citoskeleta [dissertation]. Saint Petersburg; 2017. 23 р. (In Russ.)]
- Приказ Минздрава СССР от 12.08.1977. № 755 «О мерах по дальнейшему совершенствованию форм работы с использованием экспериментальных животных». – М., 1977. [Order USSR Ministry No. 755 “O merakh po dal’nejshemu sovershenstvovaniyu form raboty s ispol’zovaniem eksperimental’nykh zhivotnykh”. Moscow; 1977. (In Russ.)]. Доступно по: http://primatologia.ru/images/NII/GLP/3_2_prikaz_minzdrawa_o_merah_zhiwotnyh.pdf. Ссылка активна на 12.04.2019.
- Стальная И.Д. Метод определения диеновой конъюгации ненасыщенных жирных кислот // Современные методы в биохимии / Под ред. В.Н. Ореховича. – М.: Медицина, 1977. – С. 63–64. [Stal’naya ID. Metod opredeleniya dienovoi kon’yugacii nenasyshchennyh zhirnyh kislot. In: Sovremennye metody v biohimii. Ed by V.N. Orekhovich. Moscow: Medicina; 1977. Р. 63–64. (In Russ.)]
- Суханов Д.С., Оковитый С.В. Гепатотропные средства в терапии поражений печени противотуберкулезными препаратами. – СПб.: Тактик-Студио, 2012. – 60 с. [Suhanov DS, Okovityj SV. Gepatotropnye sredstva v terapii porazhenii pecheni protivotuberkulyoznymi preparatami. Saint Petersburg: Taktik-Studio; 2012. 60 р. (In Russ.)]
- Халютин Д.А., Игнатенко Т.Т., Кузнецова А.К., Чайкина М.А. Оценка эффективности моликсана при острой интоксикации метанолом по критерию выживаемости / Всероссийская научно-практическая конференция, посвященная 55-летию ФГУП «НИИ ГПЭЧ» ФМБА России «Медико-биологические проблемы обеспечения химической безопасности РФ»: тез. докл. – СПб., 2017. – С. 267–268. [Halyutin DA, Ignatenko TT, Kuznecova AK, Chajkina MA. Ocenka ehffektivnosti moliksana pri ostroi intoksikacii metanolom po kriteriyu vyzhivaemosti. Vserossiiskaya nauchno-prakticheskaya konferenciya, posvyashchyonnaya 55-letiyu NII GPEHCH FMBA Rossii “Mediko-biologicheskie problemy obespecheniya himicheskoi bezopasnosti RF”: tez. dokl. Saint Petersburg; 2017. Р. 267–268. (In Russ.)]
- Gaude GS, Chaudhury A, Hattiholi J. Drug-induced hepatitis and the risk factors for liver injury in pulmonary tuberculosis patients. J Family Med Prim Care. 2015;4(2):238-243. https://doi.org/10.4103/2249-4863.154661.
- Michara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1):271-278. https://doi.org/10.1016/0003-2697(78)90342-1.
