Evaluation of the effect of Cassia fistula L. extracts on the muscle contraction intensity using an ex vivo model

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Abstract

Cassia fistula L. is a Fabaceae representative that has long been used in traditional medicine. The purpose of this study is to evaluate the effect of Cassia fistula L. herbal extracts in the form of solutions of a certain concentration on the smooth muscles of the intestine of the animal. The design of the experiment involved the identification of raw materials, their drying, grinding, extraction of soluble fractions, purification of aqueous, ethereal (diethyl ether) and ethanol extracts and their testing on the tissues of the ileum of the domestic chicken (Gallus gallus domesticus L.) extracted ex vivo.

The ethereal, alcoholic and aqueous extracts of Cassia fistula L. fruits showed to exhibit high relaxation activity compared to the control relaxation stimulants, whereas the leaf extracts showed a more modest relaxing activity. A similar situation was observed in testing extracts of young shoots, with aqueous extracts showing even more modest results, while alcohol and ethanol extracts of young shoots performed better than the corresponding leaf extracts, and the most modest results in terms of a dose sufficient for a physiological response was demonstrated by root extracts.

The initial assessment of the activity of Cassia fistula L. extracts makes it possible to identify as the most promising for further chemical study the pools of substances concentrated in the ethanol fruits extract exhibiting the minimum effective dose, in the ether extracts of fruits and bark demonstrating the shortest reaction time, and in the aqueous extracts of young shoots and cortex showing the highest percentage increase in the activity compared to the control.

About the authors

Kiran Ramchandra Ranadive

Annasaheb Magar Mahavidyalaya College

Author for correspondence.
Email: ranadive.kiran@gmail.com
ORCID iD: 0000-0001-9673-7872
Scopus Author ID: 36629222300

M.Sc., Ph.D., Associate Professor, Department of Botany

India, Hadapsar

Neeta Vijayrao Jagtap

Waghira College

Email: neetuvj7@gmail.com

M. Sci. and M. Phil., Assistant Professor, Department of Chemistry

India, Saswad

Pradnya Nilesh Jagtap

SGRS P.D.E.A. College of Pharmacy

Email: pnj1511@gmail.com

M. Pharm., Assistant Professor, Department of Pharmacology

India, Saswad

Vladimir V. Perelygin

Saint Petersburg State Chemical and Pharmaceutical University

Email: vladimir.pereligin@pharminnotech.com
ORCID iD: 0000-0002-0999-5644
SPIN-code: 3128-7451
ResearcherId: AAV-6556-2020

Doctor of Medicine (MD), Professor, Head of the Industrial Ecology Department

Russian Federation, St. Petersburg

Mikhail V. Zharikov

Saint Petersburg State Chemical and Pharmaceutical University

Email: zharikov.mihail@pharminnotech.com
ORCID iD: 0000-0003-0720-501X
SPIN-code: 7818-7228
ResearcherId: AAS-9156-2021

Master’s Degree Student of the Department of Industrial Ecology

Russian Federation, St. Petersburg

Ivan V. Zmitrovich

Komarov Botanical Institute of the Russian Academy of Sciences

Email: iv_zmitrovich@mail.ru
ORCID iD: 0000-0002-3927-2527
SPIN-code: 4155-3190
Scopus Author ID: 56521442400
ResearcherId: I-1523-2013
https://binran.ru/sotrudniki/4926/

D.Sc. in Biology, Leading Researcher, Laboratory of Systematics and Geography of the Fungi

Russian Federation, St. Petersburg

Gaikwad Dattatray Sanjay

College of Arts, Commerce and Science

Email: gsanjayin@yahoo.com

M. Pharmacology, Associate Professor, Department of Chemistry

India, Vagoli, Pune

References

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2. Fig. 1. Physiological response time corresponding to the optimal value of the effective dose of extracts

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3. Fig. 2. Dependence of amplitude of muscle relaxation (mm) on the dose of control preparations and extracts of Cassia fistula L. (mg), namely, aqueous (abbreviation A in the legend), ethanol (EN) and ether (E) ones of various parts of the plant, namely, fruits (abbreviation F in the legend), leaves (L), shoots (S), bark (B): 1 – EL; 2 – EL; 3 – AL; 4 – ENL; 5 – ENL; 6 – EF; 7 – EF; 8 – ENF; 9 – ENF; 10 – AF; 11 – AF;12 – ES; 13 – ES; 14 – ENS; 15 – ENS; 16 – AS; 17 – AS; 18 – EB; 19 – EB; 20 – AB; 21 – AB; 22 – ENB; 23 – ENB

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4. Fig. 3. Graph of the activity of extracts of Cassia fistula L. (% increase in activity compared to the control)

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Copyright (c) 2022 Ранадив К.Р., Джагтап Н.В., Джагтап П.Н., Перелыгин В.В., Жариков М.В., Змитрович И.В., Санджай Г.Д.

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
 


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