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Vol 13, No 2 (2013)
- Year: 2013
- Articles: 10
- URL: https://journals.rcsi.science/MAJ/issue/view/586
Articles
100 YEARS OF THE RESEARCH LIBRARY OF THE INSTITUTE OF EXPERIMENTAL MEDICINE
Medical academic journal. 2013;13(2):7-14
7-14
BETULENOL DERIVATIVES AS POTENTIAL ANTI-HIV AGENTS
Abstract
In this review, the important advances in the study of anti-HIV activity of betulenol, its natural and semisynthetic analogues obtained since 1993 are described. The perspective structures with potent anti-HIV activity are demonstrated. The structure-activity relationships of the obtained compounds are discussed. The latest trends in the development of anti-HIV agents based on the chemical modification of betulenol are underlined. The mechanisms of action of the most important agents are summarized in the review. Betulenol derivatives should be considered as a new effective class of specific inhibitors of HIV.
Medical academic journal. 2013;13(2):15-32
15-32
THE ROLE OF DISPERSIVE MEDIUM IN ANTITUMOR EFFECT OF FERROMAGNETIC IMPLANT
Abstract
The role of dispersive medium (physiological solution, light mineral oil, and ester-modified olive oil) was estimated in antitumor effect of ferromagnetic implant in SHR mice with intramuscular LIO-1 lymphosarcoma. Regardless of media used for implantation of barium hexaferrite, animals with LIO-1 showed mild antitumor effect and increase in life-span of SHR mice.
Medical academic journal. 2013;13(2):33-38
33-38
COMPLEX OF CERULOPLASMIN AND LACTOFERRIN IN HUMAN LACRIMAL FLUID
Abstract
Samples of lacrimal fluid obtained from 20 volunteers were studied to reveal the complex formed by two proteins i.e. ceruloplasmin (CP) and lactoferrin (LF). In all samples studied the complex CP/LF was revealed and its principal physico-chemical features coincided with the characteristics of such complex modeled in vitro or found in blood plasma and breast milk. It is shown that both polycationic molecules (DNA, lipopolysaccharides and heparin) and polycations (protamine, polylysine and fragment 29-38 from PACAP38 neuropeptide) cause dissociation of the CP/LF complex. Lysozyme and lipocalin that react with LF in lacrimal fluid did not prevent the formation of the CP/LF complex. Stoichiometry of the partner proteins in the CP/LF complex purified by chromatography was 1:1.
Medical academic journal. 2013;13(2):39-43
39-43
GENETIC PROFILE OF THERAPY-RESISTANT ASTHMA
Abstract
Therapy resistant bronchial asthma is the most difficult for treatment and has the most unpredictable course. In spite of the fact that multiple pathogenetic mechanisms of asthma had been studied, mechanisms of therapy resistant asthma still remain unclear. Moreover, this problem seems to be not well investigated. Up to present time there is no exact definition of the therapy resistant bronchial asthma phenotype. Also, the term therapy resistant asthma is not widely used. The aim of study was to evaluate the role of pharmacogenetic factors in glucocorticosteroid and beta2-agonists treatment in therapy resistant bronchial asthma. Also the investigation was aimed on working out of the prognostic criteria for treatment resistance taking into attention of genetic markers. In present work the results of investigation are discussed. These results gave possibility to determine the phenotype of therapy resistant bronchial asthma taking into attention pathogenetic and clinical heterogeneity. The results of study also gave possibility to find out the predictors of the disease and to work out the diagnostic criteria based on clinical peculiarities and genetic determinants.
Medical academic journal. 2013;13(2):44-50
44-50
ESTIMATION OF BACKGROUND AND JET EEG PATTERNS AT CHILDREN WITH THE MINIMUM BRAIN DYSFUNCTIONS
Abstract
Brain research of 58 children with the minimum brain dysfunctions as a result antenatal and postnatal defects CNS has revealed certain changes in EEG at 83% of children. These changes were characterised by immaturity of brain structures with delay of functional communications formation between these structures for 26% of child, irritation of a brain deep structures at level of oral brain departments with increase of processes of excitation — for 57% of children. Indirect signs of intracranial hypertensia have been revealed at 52% of all surveyed children.
Medical academic journal. 2013;13(2):51-58
51-58
HEALTH STATUS AND SOME GENOTYPIC FEATURES OF BA MOTHERS' BORN KIDS
Abstract
117 BA pregnant women and their kids were followed up and treated within 5 to 10 years. Kids' health status was estimated up to end of follow up. Аllergic pathology level within BA mothers' born kids was significantly higher, than in whole population (52,9% & 25%). Main risk factors of allergic pathology appearance within first 7 years of life are: allergic diseases in both parents (p=0,08), infantile phenotype of BA in kids' mother, (p<0,05), weak genotype of IL-4 (-590 C/T), IL-4R (Q576R), TNF-α (-308G>A) genes. Molecular-genetic study of atopic causal genes could be recommended for allergic risk screening in kids in order to perform further diagnostics and prophylaxis.
Medical academic journal. 2013;13(2):59-63
59-63
CONSTRUCTION AND TESTING OF LIFE VACCINE BASED ON THE PROBIOTIC STRAIN ENTEROCOCCUS FAECIUM L3 AS MEANS FOR PREVENTION OF VAGINAL INFECTION CAUSED BY STREPTOCOCCUS AGALACTIAE
Abstract
The paper presents the data on construction and evaluation of immunogenic and protective features of the new life vaccine against Streptococcus agalactiae (Group B streptococcus). For the construction of the vaccine a fragment of the Streptococcus agalactiae bac gene have been inserted into the chromosome of the probiotic strain. As a result the probiotic strain eхpressing immunogenic recombinant polypeptide on the cell surface was constructed. Application of the life vaccine in the vaginal cavity of mice induced local and systemic immune response and provided protection against lethal S. agalactiae infection.
Medical academic journal. 2013;13(2):64-70
64-70
MORPHOLOGICAL STUDY OF THE PANCREAS IN PATIENTS WITH CHRONIC PANCREATITIS USING IMMUNOHISTOCHEMICAL MARKERS
Abstract
An immunohistochemical study of biopsy samples obtained from 7 patients with chronic pancreatitis were performed using reactions to neurofilaments (NF), synaptophysin (Syn), chromogranin (CgA), myelin basic protein (MBP), and protein gene product 9.5 (PGP 9.5). Results obtained demonstrated high selectivity for revealing the endocrine cells of the islets of Langerhans for three markers: Syn, CgA, and PGP 9.5. In all the cases under investigation, the head of pancreas exhibits significant dystrophic and degenerative changes in neurons of intramural ganglia, nervous stems and bundles, demyelination of nervous fibers, destructive changes in epi-, peri-, and endoneurial sheaths, decrease or total loss of Syn-immunopositive efferent terminals around blood vessels and pancreatic ducts, denervation of glandular tissue of the exocrine and endocrine parts. Besides impairment of innervation, dystrophic changes and reorganization of the islets of Langerhans accompanied by migration and dedifferentiation of various kinds of endocrinocytes were found. In some cases, reaction to PGP 9.5 revealed an increased number of immunopositive nervous bundles, nervous fibers and their terminal branches in the hypertrophic connective tissue, around vessels, and inside or around inflammation infiltrates. It is suggested that PGP 9.5-immunopositive fibers are (nociceptive) afferent receptor apparatuses perceiving pain. The found inflammation infiltrates with a lot of plasmatic cells selectively clustered around the nervous ganglia, nervous trunks and bundles as well as disintegration of myelin sheaths revealed using MBP reaction in pancreatic nerves suggest possible development of autoimmune processes.
Medical academic journal. 2013;13(2):71-77
71-77
JUBILEE
Medical academic journal. 2013;13(2):78-79
78-79