Enviar artigo por via de e-mail BARNASE-BARSTAR SPECIFIC INTERACTION REGULATES CAR-T CELLS CYTOTOXIC ACTIVITY TOWARD MALIGNANCY
- Autores: Kalinin R.1, Gabibov A.1,2, Gnuchev N.3, Khurs E.3, Knorre V.1, Terekhov S.1,2, Maschan M.4, Chernov A.1, Telegin G.1, Belogurov A.1,2, Moysenovich .2, Yaroshevich I.2, Volkov D.1, Konovalova E.1, Schulga .1, Ukrainskay V.1,4, Rubtsov Y.1, Shipunova V.1, Deyev S.1,5
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Afiliações:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- M.V. Lomonosov Moscow State University
- Engelhardt Institute of molecular Biology, Russian Academy of Sciences
- Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology, and Immunology
- National Research University Higher School of Economics
- Edição: Volume 510, Nº 1 (2023)
- Páginas: 231-235
- Seção: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/135688
- DOI: https://doi.org/10.31857/S2686738922600972
- EDN: https://elibrary.ru/QFANKV
- ID: 135688
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Resumo
The development of CAR-T specific therapy made a revolution in modern oncology. Despite the pronounced therapeutic effects, this novel approach displayed several crucial limitations caused by the complications in pharmacokinetics and pharmacodynamics controls. The presence of the several severe medical complications of CAR-T therapy initiated a set of attempts aimed to regulate their activity in vivo. We propose to apply the barnase-barstar system to control the cytotoxic antitumor activity of CAR-T cells. To menage the regulation targeting effect of the system we propose to use barstar-modified CAR -T cells together with barnase-based molecules. Barnase was fused with designed ankyrin repeat proteins (DARPins) specific to tumor antigens HER2 (human epidermal growth factor receptor 2) The application of the system demonstrates the pronounced regulatory effects of CAR-T targeting.
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Sobre autores
Roman Kalinin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Autor responsável pela correspondência
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Alexander Gabibov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M.V. Lomonosov Moscow State University
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow; Russian Federation, Moscow
Nikolay Gnuchev
Engelhardt Institute of molecular Biology, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Elena Khurs
Engelhardt Institute of molecular Biology, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Vera Knorre
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Stanislav Terekhov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M.V. Lomonosov Moscow State University
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow; Russian Federation, Moscow
Mikhail Maschan
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology, and Immunology
Email: Roman.Kalinin.93@yandex.ru
Russian Federation,
Moscow
Alexandr Chernov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Georgij Telegin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Alexey Belogurov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; M.V. Lomonosov Moscow State University
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow; Russian Federation, Moscow
Anastasiia Moysenovich
M.V. Lomonosov Moscow State University
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Igor Yaroshevich
M.V. Lomonosov Moscow State University
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Dmitry Volkov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Elena Konovalova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Alexey Schulga
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Valeria Ukrainskay
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology, and Immunology
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow; Russian Federation,
Moscow
Yuri Rubtsov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Victoria Shipunova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow
Sergey Deyev
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; National Research University Higher School of Economics
Email: Roman.Kalinin.93@yandex.ru
Russian Federation, Moscow; Russian Federation, Moscow
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