Мақаланы E-mail арқылы жіберу Evolutionarily conserved DHX9/MLE helicase is involved in the regulation of its own mRNA expression level in Drosophila melanogaster
- Авторлар: Zolin I.A.1, Georgieva S.G.1, Nikolenko J.V.1
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Мекемелер:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Шығарылым: Том 520, № 1 (2025)
- Беттер: 63-67
- Бөлім: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/287116
- DOI: https://doi.org/10.31857/S2686738925010116
- EDN: https://elibrary.ru/tcnece
- ID: 287116
Дәйексөз келтіру
Ашық рұқсат
Рұқсат берілді
Аннотация
The MLE helicase of D. melanogaster, like its ortholog DHX9 in mammals, is involved in a wide range of processes related to the regulation of gene expression. In the present study, we investigated the impact of the mle[9] mutation on its own mRNA expression level. It was shown that in addition to the previously described deletion in the catalytic domain of the protein, which impairs its helicase activity, the mle[9] mutation contains an additional small deletion in the C-terminal domain. In the mle[9] mutation background, there was a threefold increase in the expression of the main transcript of the mle gene encoding the full-length protein. Binding of MLE to chromatin at the coding region and promoters of the mle gene and nearby enhancers was analyzed. To exclude the influence of dosage compensation, experiments were performed on females. The data obtained indicate the role of MLE in specific regulation of its own mRNA expression level in vivo at the adult stage.
Негізгі сөздер
Толық мәтін
Авторлар туралы
I. Zolin
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: julia.v.nikolenko@gmail.com
Ресей, Moscow
S. Georgieva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: julia.v.nikolenko@gmail.com
Academician of the RAS
Ресей, MoscowJ. Nikolenko
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: julia.v.nikolenko@gmail.com
Ресей, Moscow
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Fig. 1. The mle gene and the domain structure of the MLE protein under normal conditions and against the background of the mle[9] mutation (quoted from [11] with modifications). a – schematic representation of the mle gene and its transcripts: mle-RA is the transcript encoding the full-length protein, mle-RC is the alternative transcript. Exons are indicated by rectangles, the coding region is highlighted in gray. Arrows 1, 2, 3 indicate the position of the primers used to measure the transcription level of mle-RA and mle-RC. Arrows above the gene indicate the position of deletions in the mle[9] mutation: gray – the previously described deletion in the catalytic “core”, black – the deletion in the C-terminal domain discovered in the present study. Asterisks indicate the position of the nearby enhancers STARR_OSC-4142 and STARR_OSC-2528. b – domain structure of the MLE protein. Arrows indicate the positions of deletions in the mle[9] mutation: gray – previously described deletion in the catalytic core, black – 7 aa deletion in the C-terminal domain discovered in the present study. Regions corresponding to specific domains are shown in gray, linker regions are shown in white. Shown are two dsRNA-binding domains – dsRBD1 and ds-RBD2, minimal transactivation domain MTAD, catalytic core consisting of RecA1 and RecA2 domains and helicase-associated domain HA2, oligosaccharide/oligonucleotide binding region OB-fold, C-terminal domain RGG-box including nuclear localization signal NLS and glycine-rich sequence Gly. Conserved motifs in the catalytic core are indicated by Roman numerals.
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Fig. 2. Study of the effect of MLE on the expression of its own gene in vivo in D. melanogaster females at the imago stage. a – changes in the expression level of the mle gene transcripts against the background of the mle mutation[9]. The level of mle transcripts was measured relative to the level of the control gene Rpl32 transcript. The mle-RA value in wild-type females in one of three experimental replicates was taken as 1 on the ordinate axis. * – p<0.01. b – analysis of binding of the wild-type MLE protein (mle+) and the mutant protein (mle[9]) to the promoters of their own gene, to coding regions in the 5’-region of the gene (+460 – distance in bp from the mle-RC transcription start site) and in the 3’-region of the gene (+4470 – distance in bp from the mle-RC transcription start site) and to nearby enhancers (STARR_OSC-4142 and STARR_OSC-2528).
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