Email this article Identification of ultrastructural details of the astrocyte process system in neural tissue of the brain using correlative scanning probe and transmission electron microscopy
- Authors: Agapova О.I.1, Efimov А.Е.1, Obraztsova E.A.2,3, Mochalov K.E.2, Solovyeva D.O.2, Oleinikov V.A.2,4, Agapov I.I.1, Gautier S.V.1,5
-
Affiliations:
- Shumakov National Medical Research Center of Transplantology and Artificial Organs
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Moscow Institute of Physics and Technology
- National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
- Sechenov University
- Issue: Vol 515, No 1 (2024)
- Pages: 76-80
- Section: Articles
- URL: https://journals.rcsi.science/2686-7389/article/view/262832
- DOI: https://doi.org/10.31857/S2686738924020146
- EDN: https://elibrary.ru/WEXZZQ
- ID: 262832
Cite item
Open Access
Access granted
Abstract
Nanoscale morphological features of branched processes of glial cells may be of decisive importance for neuron-astrocytic interactions in health and disease. The paper presents the results of a correlation analysis of images of thin processes of astrocytes in the nervous tissue of the mouse brain, obtained by scanning probe microscopy and transmission electron microscopy with high spatial resolution. Samples were prepared and imaged using a unique hardware combination of ultramicrotomy and scanning probe microscopy. It was shown that the images identified details of astrocytes with a thickness of the order of tens of nanometers, which can be used in the future to reconstruct the three-dimensional structure of astrocytic processes by integrating a series of sequential images of ultrathin sections of nervous tissue.
Full Text
About the authors
О. I. Agapova
Shumakov National Medical Research Center of Transplantology and Artificial Organs
Email: igor.agapov@gmail.com
Russian Federation, Moscow
А. Е. Efimov
Shumakov National Medical Research Center of Transplantology and Artificial Organs
Email: igor.agapov@gmail.com
Russian Federation, Moscow
E. A. Obraztsova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Moscow Institute of Physics and Technology
Email: igor.agapov@gmail.com
Russian Federation, Moscow; Dolgoprudny
K. E. Mochalov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor.agapov@gmail.com
Russian Federation, Moscow
D. O. Solovyeva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: igor.agapov@gmail.com
Russian Federation, Moscow
V. A. Oleinikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
Email: igor.agapov@gmail.com
Russian Federation, Moscow; Moscow
I. I. Agapov
Shumakov National Medical Research Center of Transplantology and Artificial Organs
Author for correspondence.
Email: igor.agapov@gmail.com
Russian Federation, Moscow
S. V. Gautier
Shumakov National Medical Research Center of Transplantology and Artificial Organs; Sechenov University
Email: igor.agapov@gmail.com
Academician
Russian Federation, Moscow; MoscowReferences
- Zuo Y.-X., Jiang R. T., Zhou B. Astrocyte morphology: Diversity, plasticity, and role in neurological diseases // CNS Neuroscience & Therapeutics. 2019. V. 25. P. 661–807.
- Kiyoshi C.M., Aten S., Arzola E.P., et al. Ultrastructural view of astrocyte-astrocyte and astrocytesynapse contacts within the hippocampus // bioRxiv. 2020.
- Morita M. Modern Microscopic Approaches to Astrocytes // Int. J. Mol. Sci. 2023. V. 24. P. 5883.
- Curry N., Ghézali G., Kaminski Schierle G.S., et al. Correlative STED and Atomic Force Microscopy on Live Astrocytes Reveals Plasticity of Cytoskeletal Structure and Membrane Physical Properties during Polarized Migration // Front. Cell. Neurosci. 2017. V. 11. P. 104.
- Kikuchi T., Gonzalez-Soriano J., Kastanauskaite A., et al. Volume Electron Microscopy Study of the Relationship Between Synapses and Astrocytes in the Developing Rat Somatosensory Cortex // Cerebral Cortex. 2020. V. 30. P. 3800–3819.
- Efimov A.E., Agapov I.I., Agapova O.I., et al. A novel design of a scanning probe microscope integrated with an ultramicrotome for serial block-face nanotomography // Review of Scientific Instruments. 2017. V. 88. P. 023701.
- Агапова О.И., Ефимов А.Е., Сафонова Л.А. и др. Сканирующая оптическо-зондовая нанотомография для исследования структуры биоматериалов и клеток // Доклады Российской академии наук. Науки о жизни. 2021. T. 500. № 1. C. 483–487.
- Mochalov K.E., Chistyakov A.A., Solovyeva D.O., et al. An instrumental approach to combining confocal microspectroscopy and 3D scanning probe nanotomography // Ultramicroscopy. 2017. V. 182. P. 118–123.
- Миронов А.А., Комиссарчик Я.Ю., Миронов В.А. Методы электронной микроскопии в биологии и медицине / А.А. Миронов, Я.Ю. Комиссарчик, В.А. Миронов; отв. ред. Н.Н. Никольский. – СПб.: Наука, 1994.
- Hanaichi T., Sato T., Iwamoto T., et al. A Stable Lead by Modification of Sato’s Method // Journal of Electron Microscopy. 1986. V. 35. P. 304–306.
Supplementary files
