CBP AND RAD21 BIND AT THE TERMINI OF FORUM DOMAINS IN HUMAN CHROMOSOMES

N. A. Tchurikov; Чуриков Н. А.; I. R. Alembekov; Алембеков И. Р.; E. S. Klushevskaya; Клушевская Е. С.; P. B. Meilakh; Мейлах П. Б.; A. N. Kretova; Кретова А. Н.; O. D. Managrova; Манагарова О. Д.; G. I. Kravatskaya; Кравацкая Г. И.; Y. V. Kravatsky; Кравацкий Ю. В.

doi:10.31857/S2686738923600619

CBP AND RAD21 BIND AT THE TERMINI OF FORUM DOMAINS IN HUMAN CHROMOSOMES

Authors: Tchurikov N.A.¹, Alembekov I.R.¹, Klushevskaya E.S.¹, Meilakh P.B.¹, Kretova A.N.¹, Managrova O.D.¹, Kravatskaya G.I.¹, Kravatsky Y.V.¹
Affiliations:
1. Engelhardt Institute of Molecular Biology Russian Academy of Sciences
Issue: Vol 513, No 1 (2023)
Pages: 595-598
Section: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/244401
DOI: https://doi.org/10.31857/S2686738923600619
EDN: https://elibrary.ru/XZIWQN
ID: 244401

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Abstract
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Abstract

Forum-domains are 50–100-kb stretches of DNA delimited by the hot sport of DSBs. These domains possess coordinately expressed genes. However, molecular mechanisms of such regulation are not clear. It is supposed that the proteins specifically binding at the termini of domains could be involved in coordinated regulation of expression. In this study we used the results of precise mapping of hot spots of DSBs and ChIP-Seq data for ten nuclear proteins in HEK293T cell line for a search of proteins specifically binding at forum-domains termini. We detected that two proteins – CBP and RAD24-, which are known to be involved in epigenetic regulation of gene expression and formation of 3D chromosomal structures, bind at the termini. We suppose that these proteins could be involved in coordinated expression of genes in forum-domains.

Keywords

forum-domains, DSBs, epigenetics, CBP, RAD21

References

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