DIFFERENT PROTEIN GROUPS INVOLVED IN TRANSCRIPTION REGULATION IN GENES OF DEVELOPMENT AND HOUSEKEEPING IN  DROSOPHILA

I. F. Zhimulev; Жимулев И. Ф.; T. Yu. Vatolina; Ватолина Т. Ю.; G. V. Pokholkova; Похолкова Г. В.; O. V. Antonenko; Антоненко О. В.; M. V. Maltseva; Мальцева М. В.

doi:10.31857/S2686738923700348

DIFFERENT PROTEIN GROUPS INVOLVED IN TRANSCRIPTION REGULATION IN GENES OF DEVELOPMENT AND HOUSEKEEPING IN DROSOPHILA

Authors: Zhimulev I.F.¹, Vatolina T.Y.¹, Pokholkova G.V.¹, Antonenko O.V.¹, Maltseva M.V.¹
Affiliations:
1. Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences
Issue: Vol 512, No 1 (2023)
Pages: 438-443
Section: Articles
URL: https://journals.rcsi.science/2686-7389/article/view/140842
DOI: https://doi.org/10.31857/S2686738923700348
EDN: https://elibrary.ru/SRXVUU
ID: 140842

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

Antibodies to histone modifications and an insulator protein involved in the processes of transcription initiation and elongation are mapped in Drosophila polytene chromosomes. The CHRIZ protein (chromatin insulator) and H3K36me3 histone modification (RNA elongation) are detected only in the localization of housekeeping genes (interbands and gray bands of polytene chromosomes) and never in the regions of development genes (black bands and large puffs arising from them). Antibodies to H3S10P histone modification, which is associated with the initial elongation of the RNA strand during transcription, are found exclusively in small puffs, but not in housekeeping gene localization sites or large ecdysone-induced puffs, where housekeeping genes are localized. Antibodies to H4R3me2 histone modification (a co-repressor of the ecdysone receptor) are detected only in large α-induced puffs.

Keywords

histone modifications H3K36, H3S10P, H4R3me2, CHRIZ protein, housekeeping genes, developmental genes, gene promoters, polytene chromosomes, Drosophila

References

Lenhard B., Sandelin A., Carninci P. Metazoan promoters: emerging characteristics and insights into transcriptional regulation // Nat. Rev. Genet. 2012. V. 13. № 4. P. 233–245.
Andersson R., Sandelin A. Determinants of enhancer and promoter activities of regulatory elements // Nat. Rev. Genet. 2020. V. 21. P. 71–87.
Boros I.M. Histone modification in Drosophila // Briefings in Functional Genomics. 2012. V. 11. № 4. P. 319–331.
Lindehell H., Glotov A., Dorafshan E., et al. The role of H3K36 methylation and associated methyltransferases in chromosome-specific gene regulation // Sci. Adv. 2021. V. 7. № 40. eabh4390.
Zhimulev I.F., Zykova T.Yu., Goncharov F.P., et al. Genetic organization of polytene chromosome bands and interbands in Drosophila melanogaster // PLoS One. 2014. V. 9. № 7. P. e101631.
Bridges C.B. A revised map of the salivary gland X-chromosome of Drosophila melanogaster // J. Hered. 1938. V. 29. № 1. P. 11–13.
Kozlova T.Yu., Semeshin V.F., Tretyakova I.V., et al. Molecular and cytogenetical characterization of the 10A1-2 band and adjoining region in the Drosophila melanogaster polytene X chromosome // Genetics. 1994. V. 136. № 3. P. 1063–1073.
Zykova T.Yu., Levitsky V.G., Belyaeva E.S., et al. Polytene chromosomes – a portrait of functional organization of the Drosophila genome // Curr. Genomics. 2018. V.19. № 3. P. 179–191.
Ashburner M., Chihara C., Meltzer P., et al. Temporal control of puffing activity in polytene chromosomes // Cold Spring Harbor Symp. Quant. Biol. 1974. V. 38. P. 655–662.
Zhimulev I.F. Genetic organization of polytene chromosomes // Advances in Genetics. 1999. V. 39. P. 1–589.
Thummel C.S. Ecdysone-regulated puff genes 2000 // Insect Biochem. Mol. Biol. 2002 V. 32. № 2. P. 113–20.
King-Jones K., Thummel C. Nuclear receptors – a perspective from Drosophila // Nat. Rev. Genet. 2005. V. 6. № 4. P. 311–323.
FlyBase version FB2022_06, released Desember 13, 2022.
Pokholkova G.V., Demakov S.A., Andreenkov O.V., et al. Tethering of CHROMATOR and dCTCF proteins results in decompaction of condensed bands in the Drosophila melanogaster polytene chromosomes but does not affect their transcription and replication timing // Plos One. 2018. V. 13. № 4. P. e0192634.
Boldyreva L.V., Goncharov F.P., Demakova O.V., et al. Protein and genetic composition of four chromatin types in Drosophila melanogaster Cell Lines // Curr. Genomics. 2017. V. 18. № 2. P. 214–226.
Sharda A., Humphrey T.C. The role of histone H3K36me3 writers, readers and erasers in maintaining genome stability // DNA Repair. 2022. № 119: 103407.
Kimura S., Sawatsubashi S., Ito S., et al. Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor. Biochem. Biophys. Res. Commun. 2008. V. 371. № 4. P. 889–893.
Karam C.S., Kellner W.A., Takenaka N., et al. 14-3-3 mediates histone cross-talk during transcription elongation in Drosophila // PLoS Genetics. 2010. V. 6. № 6. P. e1000975.
Cai W., Bao X., Deng H., et al. RNA polymerase II-mediated transcription at active loci does not require histone H3S10 phosphorylation in Drosophila. Development. 2008. V. 135. № 17. P. 2917–2925.