Influence of solar radiation on chlorophyll a concentration assessment using fluorescence measured by the submersible sensor in Lake Baikal
- Authors: Moiseeva N.A.1, Efimova Т.V.1, Churilova Т.Y.1, Makarov М.М.2
-
Affiliations:
- Kovalevski Institute of Biology of the Southern Seas, Russian Academy of Sciences
- Limnological Institute of the Siberian Branch of the Russian Academy of Sciences
- Issue: No 4 (2019)
- Pages: 281-285
- Section: Articles
- URL: https://journals.rcsi.science/2658-3518/article/view/288702
- DOI: https://doi.org/10.31951/2658-3518-2019-A-4-281
- ID: 288702
Cite item
Full Text
Abstract
Assessment of chlorophyll a concentration based on fluorescence intensity is actively used at present. In natural waters, profile fluorescence is measured using submersible sensors. These sensors are equipped with no special chamber for phytoplankton dark adaptation before measurement. Effect of irradiance in the upper layer leads to a decrease in chlorophyll a fluorescence due to closing some reaction centers of photosystem 2. The conducted research on Lake Baikal has revealed the relationship between the share of open reaction center in photosystem 2 and photosynthetically available radiation in the lake. The relationship between these parameters was described by an exponential function with a high determination coefficient (r2=0.97). Based on the obtained relationship, an algorithm was developed to compensate for the decrease in chlorophyll a fluorescence intensity due to the light influence in situ. The algorithm enables to retrieve the “real” fluorescence profile, which is necessary for the correct retrieval of the vertical distribution of the chlorophyll a content.
About the authors
N. A. Moiseeva
Kovalevski Institute of Biology of the Southern Seas, Russian Academy of Sciences
Author for correspondence.
Email: nataliya-moiseeva@yandex.ru
Russian Federation, 2 Nakhimov Ave, Sevastopol, 299011
Т. V. Efimova
Kovalevski Institute of Biology of the Southern Seas, Russian Academy of Sciences
Email: nataliya-moiseeva@yandex.ru
Russian Federation, 2 Nakhimov Ave, Sevastopol, 299011
Т. Y. Churilova
Kovalevski Institute of Biology of the Southern Seas, Russian Academy of Sciences
Email: nataliya-moiseeva@yandex.ru
Russian Federation, 2 Nakhimov Ave, Sevastopol, 299011
М. М. Makarov
Limnological Institute of the Siberian Branch of the Russian Academy of Sciences
Email: nataliya-moiseeva@yandex.ru
Russian Federation, Ulan-Batorskaya 3, 664 033 Irkutsk
References
- Babin M. 2008. Phytoplankton fluorescence: theory, current literature and in situ measurement. In: Babin M., Roesler C., Cullen J. (Eds.), Real-time coastal observing systems for marine ecosystem dynamics and harmful algal blooms. Paris: UNESCO Publishing, pp. 237-281.
- Barbieux M., Uitz J., Gentili B. et al. 2019. Bio-optical characterization of subsurface chlorophyll maxima in the Mediterranean Sea from a Biogeochemical-Argo float database. Biogeosciences 16: 1321-1342. doi: 10.5194/bg-16-1321-2019
- Falkowski P., Kiefer D.A. 1985. Chlorophyll a fluorescence in phytoplankton: relationship to photosynthesis and biomass. Journal of Plankton Research 7: 715-731. doi: 10.1093/plankt/7.5.715
- Falkowski P.G., Raven J.A. 2007. Aquatic photosynthesis. Princeton and Oxford: Princeton University Press.
- Gaevsky N.A., Morgun V.N. 1993. Using variable and slow chlorophyll fluorescence to study plant photosynthesis. Fiziologija Rastenij [Plant physiology] 40: 119-127. (in Russian)
- Govindjee M., Van de Ven C., Preston C. et al. 1990. Chlorophyll a fluorescence lifetime distributions in open and closed photosystem II reaction center preparations. Biochimica et Biophysica Acta 1015: 173-179. doi: 10.1016/0005-2728(90)90017-X
- Kirk J.T.O. 2011. Light and photosynthesis in aquatic ecosystems. Third edition. Cambridge: Cambridge University Press.
- Konyukhov I.V., Vorobyova O.V., Pogosyan S.I. et al. 2017. Fluorometer with pulse modulation of exciting light for estimating the functional state of natural phytoplankton by chlorophyll fluorescence parameters. In: II Vserossijskaja nauchnaya konferenciya molodyh uchenyh “Kompleksnye Issledovanija Mirovogo Okeana”, pp. 345-347. (in Russian)
- Kubryakov A.A., Stanichny S.V., Kubryakova E.A. 2017. Variability of the bio-optical characteristics of the Black Sea from measurements of Bio-Argo buoys and satellite data. In: II Vserossijskaja nauchnaya konferenciya molodyh uchenyh “Kompleksnye Issledovanija Mirovogo Okeana”, pp. 130-131. (in Russian)
- Matorin D.N., Osipov V.A., Rubin A.B. 2012. Metodika izmereniy obiliya i indikatsii izmeneniya sostoyaniya fitoplanktona v prirodnykh vodakh fluorestsentnym metodom. Teoreticheskiye i prakticheskiye aspekty. Moskow: Al’teks. (in Russian)
- Moiseeva N., Churilova T., Efimova T. et al. 2018. Dependence of fluorescence intensity on chlorophyll a concentration and light absorption coefficients by phytoplankton in the Black Sea (October 2017). Proceedings of the SPIE 10833. doi: 10.1117/12.2505415
- Odermatt D., Pomati F., Pitarch J. et al. 2012. MERIS observations of phytoplankton blooms in a stratified eutrophic lake. Remote Sensing of Environment 126: 232-239. doi: 10.1016/j.rse.2012.08.031
- Pogosyan S.I., Matorin D.N. 2005. Variability in the condition of the photosynthetic system of the Black Sea phytoplankton. Oceanology 45: 139-148.
- Schreiber U., Bilger W., Neubauer C. 1995. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze E.D., Caldwell M.M. (Eds.), Ecophysiology of photosynthesis. Berlin, Heidelberg, pp. 49-70. doi: 10.1007/978-3-642-79354-7_3
- Suggett D.J., Borowitzka M.A., Prášil O. 2010. Chlorophyll a fluorescence in aquatic sciences: Methods and applications. Dordrecht: Springer. doi: 10.1007/978-90-481-9268-7
- Wojtasiewicz B., Hardman-Mountford N.J., Antoine D. et al. 2018. Use of bio-optical profiling float data in validation of ocean colour satellite products in a remote ocean region. Remote Sensing of Environment 209: 275-290. doi: 10.1016/j.rse.2018.02.057
- Xing X., Morel A., Claustre H. et al. 2012. Combined processing and mutual interpretation of radiometry and fluorometry from autonomous profiling Bio-Argo floats: 2. Colored dissolved organic matter absorption retrieval. Journal of Geophysical Research: Oceans 117. doi: 10.1029/2011jc007632
Supplementary files
