Nanostructural features of contacts of fibroblasts with dual-scale bioсompatible polyurethane scaffold
- Авторлар: Koudan E.V.1, Pereira F.D.1, Bulanova E.A.1, Mironov V.A.1, Agapov I.I.2, Efimov A.E.2, Agapova O.I.2, Safonova L.A.2, Bobrova M.M.2, Parfenov V.A.1
-
Мекемелер:
- Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
- Laboratory of Bionanotechnology, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
- Шығарылым: Том 11, № 11-12 (2016)
- Беттер: 830-834
- Бөлім: Article
- URL: https://journals.rcsi.science/2635-1676/article/view/219810
- DOI: https://doi.org/10.1134/S1995078016060094
- ID: 219810
Дәйексөз келтіру
Аннотация
This paper presents a study of nanostructural features of contacts of bioprinted tissue spheroids with polyurethane dual scale biocompatible scaffold made by three-dimensional printing and electrospinning. Analysis of nanostructural features of cell contacts was carryed out by scanning probe microscopy with use of experimental setup combining ultramicrotome and scanning probe microscope. Measured mean cell volume is 460 ± 104 μm3, mean contact area of cells with scaffold fibers–104.8 μm2 per cell (16.7% of total cell area). Maximum distance of migrating cells from spheroid border at 48 h. is ~200 μm, what corresponds to mean velocity of cell migration more than 4 μm/h. Obtained quantitative characteristics of micro- and nanostructure of human fibroblast cell contacts with elecrospun polyurethane scaffold secure high efficacy of tissue regeneration with its usage for implanted bioprinted dual scale tissue-engineered scaffolds.
Авторлар туралы
E. Koudan
Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
Email: igor_agapov@mail.ru
Ресей, Moscow, 115409
F. Pereira
Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
Email: igor_agapov@mail.ru
Ресей, Moscow, 115409
E. Bulanova
Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
Email: igor_agapov@mail.ru
Ресей, Moscow, 115409
V. Mironov
Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
Email: igor_agapov@mail.ru
Ресей, Moscow, 115409
I. Agapov
Laboratory of Bionanotechnology, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
Хат алмасуға жауапты Автор.
Email: igor_agapov@mail.ru
Ресей, Moscow, 123182
A. Efimov
Laboratory of Bionanotechnology, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: igor_agapov@mail.ru
Ресей, Moscow, 123182
O. Agapova
Laboratory of Bionanotechnology, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: igor_agapov@mail.ru
Ресей, Moscow, 123182
L. Safonova
Laboratory of Bionanotechnology, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: igor_agapov@mail.ru
Ресей, Moscow, 123182
M. Bobrova
Laboratory of Bionanotechnology, Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
Email: igor_agapov@mail.ru
Ресей, Moscow, 123182
V. Parfenov
Private Institution Laboratory for Biotechnological Research “3D Bioprinting Solutions”
Email: igor_agapov@mail.ru
Ресей, Moscow, 115409
Қосымша файлдар
