Email this article Evaluation properties of bioelectrodes based on carbon superfine materials containing model microorganisms Gluconobacter
- Authors: Reshetilov A.N.1, Plekhanova J.V.1, Tarasov S.E.1, Bykov A.G.1, Gutorov M.A.2, Alferov S.V.3, Tenchurin T.K.4, Chvalun S.N.4, Orekhov A.S.4, Shepelev A.D.4, Gotovtsev P.M.4, Vasilov R.G.4
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Affiliations:
- Skryabin Institute of Biochemistry and Physiology of Microorganisms
- OOO Gamma LLC
- Tula State University
- National Research Center “Kurchatov Institute,”
- Issue: Vol 12, No 1-2 (2017)
- Pages: 107-115
- Section: Article
- URL: https://journals.rcsi.science/2635-1676/article/view/219903
- DOI: https://doi.org/10.1134/S1995078017010098
- ID: 219903
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Abstract
We have studied the properties of a bioelectrode formed by the immobilization of Gluconobacter oxydans bacterial cells on carbon superfine materials (CSMs). We use three types of CSMs (as adopted by the working classification CSM 1–3) with different carbonization rates. The bioelectrode is formed by covering the surface of the CSM suspension of bacteria in a chitosan gel. The properties of samples are evaluated by measuring the physiological state of the bacteria immobilized: (a) recording the intensity of cellular respiration, (b) for measuring the charge transport characteristics of electrode (bioelectrocatalysis), and (c) by measuring the electrode impedance. Measurements (b) and (c) are made on two and three-electrode circuits in the oxidation of ethanol in the presence of 2,6-dichlorophenol bacteria electron transport mediator. For CSMs 1 and 2 the electron transport by the oxidation of the substrate is not registered, while for CSM 3 the current generation occurs. The resistance of CSM 3 bioelectrode is below the resistance of CSMs 1 and 2 both before (39.6 kΩ/cm2 for CSM 3, 630 Ω/cm2 for CSM 2, and 1329 Ω/cm2 for CSM 1) and after the addition of the substrate (2.9 kΩ/cm2 for CSM 3, 45 kΩ/cm2 for CSM 2, and 58 kΩ/cm2 for CSM 1). The bioelectrode made of CSM 3 has a capacitance of 196 μF/cm2—greater than two orders of magnitude of the bioelectrode capacity of CSMs 1 and 2 (0.51 and 0.58 μF/cm2, respectively). It is important to further study the properties of the CSM class of materials, which are promising as the basis of mechanically flexible electrodes with controlled parameters.
About the authors
A. N. Reshetilov
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Author for correspondence.
Email: anatol@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
J. V. Plekhanova
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: anatol@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
S. E. Tarasov
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: anatol@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
A. G. Bykov
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: anatol@ibpm.pushchino.ru
Russian Federation, Pushchino, Moscow oblast, 142290
M. A. Gutorov
OOO Gamma LLC
Email: anatol@ibpm.pushchino.ru
Russian Federation, Zelenograd, Moscow, 124498
S. V. Alferov
Tula State University
Email: anatol@ibpm.pushchino.ru
Russian Federation, Tula, 300012
T. K. Tenchurin
National Research Center “Kurchatov Institute,”
Email: anatol@ibpm.pushchino.ru
Russian Federation, Moscow, 123182
S. N. Chvalun
National Research Center “Kurchatov Institute,”
Email: anatol@ibpm.pushchino.ru
Russian Federation, Moscow, 123182
A. S. Orekhov
National Research Center “Kurchatov Institute,”
Email: anatol@ibpm.pushchino.ru
Russian Federation, Moscow, 123182
A. D. Shepelev
National Research Center “Kurchatov Institute,”
Email: anatol@ibpm.pushchino.ru
Russian Federation, Moscow, 123182
P. M. Gotovtsev
National Research Center “Kurchatov Institute,”
Email: anatol@ibpm.pushchino.ru
Russian Federation, Moscow, 123182
R. G. Vasilov
National Research Center “Kurchatov Institute,”
Email: anatol@ibpm.pushchino.ru
Russian Federation, Moscow, 123182
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