Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus
- Authors: Khalikov A.A.1, Kildyushov E.M.2, Kuznetsov K.O.1, Komlev D.S.3, Rahmatullina G.R.3
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Affiliations:
- Bashkir State Medical University
- N.I. Pirogov Russian National Research Medical University
- Volga-Ural Bureau of Forensic Medicine
- Issue: Vol 7, No 2 (2021)
- Pages: 96-100
- Section: CASE REPORTS
- URL: https://journals.rcsi.science/2411-8729/article/view/122410
- DOI: https://doi.org/10.17816/fm401
- ID: 122410
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Abstract
Background: Lymphoid tissue is the first to react to stress, which manifests as characteristic morphological manifestations, based on which it is possible to study the duration of damage. Aims: Study of the dynamics of changes in the histomorphometric characteristics of the rat thymus during regeneration of the musculocutaneous tissue during mechanical thigh trauma. Material and methods: In total, 84 sexually mature rats were enrolled. These were divided into two groups (experiment and control), which were further classified into four subgroups depending on the time elapsed since the injury (1, 3, 15, and 25 days). Mechanical damage was simulated using a setup that transferred kinetic energy (comparable to a 5.6-mm rifle bullet) to tissues. Subsequently, the animals were removed from the experiment by decapitation; their thymus was removed and microscopically examined. In arbitrary units, the total area of the lobules as well as the area of the cortical and cerebral zones was determined. Results: Our research results indicate different phases of changes in the histomorphometric characteristics of the thymus, which correspond to the course of the wound and can be used in forensic practice to determine the duration of the injury. Conclusions: Mechanical trauma, considered as a stress reaction, causes involutive changes in the thymus and is characterized by changes in the area of the thymus cortex and medulla as well as regular dynamics of changes in the thymic lobules at different stages of wound regeneration.
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##article.viewOnOriginalSite##About the authors
Airat A. Khalikov
Bashkir State Medical University
Email: airat.expert@mail.ru
ORCID iD: 0000-0003-1045-5677
SPIN-code: 1895-7300
MD, Dr. Sci. (Med.), Professor
Russian Federation, 450008, Ufa, Lenin street, 3Evgeny M. Kildyushov
N.I. Pirogov Russian National Research Medical University
Email: kem1967@bk.ru
ORCID iD: 0000-0001-7571-0312
SPIN-code: 6412-0687
MD, Dr. Sci. (Med.), Professor
Russian Federation, MoscowKirill O. Kuznetsov
Bashkir State Medical University
Author for correspondence.
Email: kirillkuznetsov@aol.com
ORCID iD: 0000-0002-2405-1801
Student
Russian Federation, 450008, Ufa, Lenin street, 3Dmitry S. Komlev
Volga-Ural Bureau of Forensic Medicine
Email: komlevds@mail.ru
ORCID iD: 0000-0003-4180-5326
expert
Russian Federation, UfaGulnaz R. Rahmatullina
Volga-Ural Bureau of Forensic Medicine
Email: rgulnaz779@gmail.ru
ORCID iD: 0000-0001-9509-5978
expert
Russian Federation, UfaReferences
- Enikeev DA, Kuznetsov KO, Enikeev OA, et al. Pathophysiological and pathochemical aspects of the effect of hydrogen peroxide on the human and animal organism. Pathogenesis. 2021;19(1):30–36. (In Russ). doi: 10.25557/2310-0435.2021.01.30-36
- Taves MD, Ashwell JD. Glucocorticoids in T cell development, differentiation and function. Nat Rev Immunol. 2021;21(4): 233–243. doi: 10.1038/s41577-020-00464-0
- Murzabaev HH, Kashapov IG. Method for dosed transfer of projectile kinetic energy to damaged tissues. Morphology. 2001; 120(6):83−84. (In Russ).
- Kondo T. Timing of skin wounds. Legal Medicine. 2007; 9(2):109–114. doi: 10.1016/j.legalmed.2006.11.009
- Rezzani R, Franco C, Hardeland R, Rodella LF. Thymus-pineal gland axis: revisiting its role in human life and ageing. Int J Mol Sci. 2020;21(22):8806. doi: 10.3390/ijms21228806
- Miller JF. The function of the thymus and its impact on modern medicine. Science. 2020;369(6503):eaba2429. doi: 10.1126/science.aba2429
- Billard MG, Gruver AL, Sempowski GD. Acute endotoxin-induced thymic atrophy is characterized by intrathymic inflammatory and wound healing responses. PLos One. 2011;6:e17940. doi: 10.1371/journal.pone.0017940
- Cosway EJ, James KD, Lucas B, et al. The thymus medulla and its control of αβT cell development. Semin Immunopathol. 2021;43(1):15–27. doi: 10.1007/s00281-020-00830-z
- Han J, Zúñiga-Pflücker JC. A 2020 View of thymus stromal cells in T cell development. J Immunol. 2021;206(2):249–256. doi: 10.4049/jimmunol.2000889
- McBride MA, Owen AM, Stothers CL, et al. The metabolic basis of immune dysfunction following sepsis and trauma. Front Immunol. 2020;29(11):1043. doi: 10.3389/fimmu.2020.01043