Effects of nitric oxide associated with expression of some genes at embryonic stage of avian development
- Authors: Titov V.Y.1,2, Kochish I.I.1, Myasnikova O.V.1, Dolgorukova A.M.2, Zaytseva M.A.1, Romanov P.S.1, Vardanyan H.R.3
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Affiliations:
- Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin
- Russian Research and Technological Poultry Institute
- Scientific Center for Food Safety Risk Assessment and Analysis
- Issue: Vol 20, No 1 (2025): Therapeutic case of animal welfare
- Pages: 151-161
- Section: Genetics and selection of animals
- URL: https://journals.rcsi.science/2312-797X/article/view/315865
- DOI: https://doi.org/10.22363/2312-797X-2025-20-1-151-161
- EDN: https://elibrary.ru/IZQTNT
- ID: 315865
Cite item
Abstract
Data obtained by various researchers indicate the effect of nitric oxide (NO) on expression of some genes, in particular, the genes associated with myogenesis in birds. But until now it has not been possible to quantify this effect and possibility of its use, since the control of content of NO metabolites in tissues presented a methodological difficulty. The purpose of this research was to find out relationship between the NO content in tissues of avian embryos and the expression of genes responsible for myogenesis at various stages of embryogenesis. We used a highly sensitive and highly specifi enzyme sensor to determine the content of the main NO metabolites in tissues. Gene expression was determined by PCR-RT. The relationship between the NO content in tissues and the expression of 7 genes involved in the process of myogenesis was studied in chicken and quail embryos. There are the genes of myocyte proliferation factor 2c (mef 2c), myogenic differentiation 1 (MyoD1), myogenesis factor 5 (myf 5), myosin (mhy 1), myogenin (myog), somatostatin (MSTN), gonadotropin hormone (GHR). Blocking the synthesis of NO, which leads to a decrease in deposited NO in tissues by 50…70%, results in a change in expression of most of the studied genes. Basically, there was a decrease in gene expression, in particular, myostatin (MSTN), which is responsible for suppressing growth and differentiation of muscle tissue. Thus, nitric oxide in an avian embryo can primarily play the role of regulator of muscle tissue growth, which is important for fast-growing breeds since myoblast proliferation occurs at embryonic stage of development. Regulation can be carried out by activating the mechanisms of oxidation of NO to nitrate which occurs in the embryos of fast-growing breeds.
Keywords
About the authors
Vladimir Y. Titov
Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin; Russian Research and Technological Poultry Institute
Author for correspondence.
Email: vtitov43@yandex.ru
ORCID iD: 0000-0002-2639-7435
SPIN-code: 3912-0315
Doctor of Biological Sciences, Professor of Department of Radiobiology and Biophysics, Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin; Chief Researcher, Russian Research and Technological Institute of Poultry Farming
23 Akademika Scriabina st., Moscow, 109472, Russian Federation; 10 Ptitsegradskaya st., Sergiev Posad, Moscow region, 141311, Russian FederationIvan I. Kochish
Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin
Email: kochish.I@mail.ru
ORCID iD: 0000-0001-8892-9858
SPIN-code: 9333-9995
Academician of the Russian Academy of Sciences, Doctor of Agricultural Sciences, Head of the Department of Animal Hygiene and Poultry Breeding named after A.K. Danilova
23 Akademika Scriabina st., Moscow, 109472, Russian FederationOlga V. Myasnikova
Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin
Email: omyasnikova71@gmail.com
ORCID iD: 0000-0002-9869-0876
SPIN-code: 1466-8393
Candidate of Agricultural Sciences, Associate Professor, Department of Animal Hygiene and Poultry Breeding named after A.K. Danilova
23 Akademika Scriabina st., Moscow, 109472, Russian FederationAnna M. Dolgorukova
Russian Research and Technological Poultry Institute
Email: anna.dolg@mail.ru
ORCID iD: 0000-0002-9958-8777
SPIN-code: 7630-3146
Candidate of Biological Sciences, Head of the Incubation Department
10 Ptitsegradskaya st., Sergiev Posad, Moscow region, 141311, Russian FederationMaria A. Zaytseva
Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin
Email: may-zay@yandex.ru
student, Faculty of Biotechnology and Ecology 23 Akademika Scriabina st., Moscow, 109472, Russian Federation
Pyotr S. Romanov
Moscow State Academy of Veterinary Medicine and Biotechnology - MVA named after K.I. Skryabin
Email: romanovpeter1367@yandex.ru
student, Faculty of Biotechnology and Ecology 23 Akademika Scriabina st., Moscow, 109472, Russian Federation
Harutyun R. Vardanyan
Scientific Center for Food Safety Risk Assessment and Analysis
Email: harvard3@yandex.ru
ORCID iD: 0009-0002-1050-8433
Candidate of Biological Sciences, Researcher
107 Masisi Shosse, bldg. 2, Yerevan, 0071, Republic of ArmeniaReferences
- Vasudevan D, Bovee RC, Thomas DD. Nitric oxide, the new architect of epigenetic landscapes. Nitric Oxide, 2016;59:54—62. doi: 10.1016/j.niox.2016.08.002 EDN: XZGAXR
- Hickok JR, Vasudevan D, Antholine WE, Thomas DD. Nitric oxide modifies global histone methylation by inhibiting jumonji C domain‑c ontaining demethylases. J Biol Chem. 2013;288(22):16004—16015. doi: 10.1074/jbc.M112.432294
- Socco S, Bovee RC, Palczewski MB, Hickok JR, Thomas DD. Epigenetics: The third pillar of nitric oxide signaling. Pharmacological Research. 2017;121:52—58. doi: 10.1016/j.phrs.2017.04.011
- Cazzato D, Assi E, Moscheni C, Brunelli S, De Palma C, Cervia D, et al. Nitric oxide drives embryonic myogenesis in chicken through the upregulation of myogenic differentiation factors. Experimental Cell Research. 2014;320(2):269—280. doi: 10.1016/j.yexcr.2013.11.006 EDN: SSFUQH
- Titov VY. The enzymatic technologies open new possibilities for studying nitric oxide (NO) metabolism in living systems. Current Enzyme Inhibition. 2011;7(1):56—70. doi: 10.2174/157340811795713774 EDN: OIAEVH
- Titov VY, Kochish II, Dolgorukova AM. Oksid azota (NO) v embrional’nom i postembrional’nom razvitii ptits [Nitric oxide (NO) in embryonic and postembryonic development of birds]. Moscow; 2022. (In Russ.). doi: 10.18720/SPBPU/2/z22—25 EDN: VSDSLT
- Titov VY, Dolgorukova AM, Kochish II, Myasnikova OV. Nitric oxide (NO) content and expression of genes involved in myogenesis in embryonal tissues of chickens (Gallus gallus domesticus L.). Agricultural biology. 2024;59(2):316—327. (In Russ.). doi: 10.15389/agrobiology.2024.2.316eng EDN: BTJQCF
- Krych‑M adej J, Gebicka L. Interactions of nitrite with catalase: Enzyme activity and reaction kinetics studies. J Inorg Biochem. 2017;171:10—17. doi: 10.1016/j.jinorgbio.2017.02.023 EDN: YYAUVP
- Schmittgen TD, Livak KJ. Analyzing real — time PCR data by comparative С(T) method. Nature Protocols. 2008;3(6):1101—1108, doi: 10.1038/nprot. 2008.73
- Rozenboim I, El Halawani ME, Kashash Y, Piestun Y, Halevy O. The effect of monochromatic photostimulation on growth and development of broiler birds. General and Comparative Endocrinology. 2013;190:214—219. doi: 10.1016/j.ygcen.2013.06.027
- Sobolewska A, Elminowska‑ Wenda G, Bogucka J, Szpinda M, Walasik K, Bednarczyk M, et al. Myogenesis — Possibilities of its Stimulation in Chickens. Folia biologica (Kraków). 2011;59(3–4):85—90. doi: 10.3409/fb59_3‑4.85‑90
- Halevy O, Piestun Y, Rozenboim I, Yablonka‑Reuveni Z. In ovo exposure to monochromatic green light promotes skeletal muscle cell proliferation and affects myofiber growth in posthatch chicks. Am J Physiol Regul Integr Comp Physiol. 2006;290(4): R1062‑R1070. doi: 10.1152/ajpregu.00378.2005
- Vanin A.F. Physico‑c hemistry of dinitrosyl iron complexes as a determinant of their biological activity. Int J Mol Sci. 2021;22(19):10356. doi: 10.3390/ijms221910356 EDN: CKMCHK
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