Antimicrobial resistance of bovine mastitis pathogens
- Authors: Firsov G.M.1, Ryadnov A.A.1, Ryadnova T.A.1, Morozova Z.C.1, Budtuev O.V.1
-
Affiliations:
- Volgograd State Agrarian University
- Issue: Vol 19, No 1 (2024): Factors of sustainable animal productivity: from genomics to therapy
- Pages: 51-60
- Section: Factors of sustainable animal productivity: from genomics to therapy
- URL: https://journals.rcsi.science/2312-797X/article/view/315827
- DOI: https://doi.org/10.22363/2312-797X-2024-19-1-51-60
- EDN: https://elibrary.ru/AMXNZZ
- ID: 315827
Cite item
Full Text
Abstract
Inflammation of mammary gland - mastitis - can be caused by various reasons, both physical and microbiological. Mastitis affects physical and technological quality of milk, growth of economic costs and increase in milk production cost, especially due to the use of antimicrobial drugs. The purpose of the research was to study antibacterial resistance of mastitis pathogens in cattle. The studies were carried out in the Oktyabrsky and Kamyshinsky districts, Volgograd region in 2019-2023. 1206 milk samples from Holstein- Friesian cows aged 4-7 years were examined using standard microbiological methods. Antimicrobial susceptibility testing was performed on Mueller - Hinton agar using a modified Kirby - Bauer disc diffusion method. Based on the results of microbiological studies, microbial growth of 984 cultures ( S. aureus , Str. agalactiae , Str. uberis , E. coli , Klebsiella spp. , Pseudomonas aeruginosa , Pasteurella multocida , Trueperella pyogenes and Mycoplasma spp. ) was identified . In the study, multiple resistance to the studied antimicrobial drugs was noted, for example, E. coli had resistance to all 9 drugs; Pseudomonas aeruginosa - to 8; Mycoplasma spp. - to 7; S. aureus , Str. agalactiae , Str. uberis , Klebsiella spp . and Pasteurella multocida - to 6; Trueperella pyogenes to 1 drug. Constant monitoring of resistance of bovine mastitis pathogens to antimicrobial agents contributes to a more effective selection of therapy for clinical mastitis.
About the authors
Grigory M. Firsov
Volgograd State Agrarian University
Author for correspondence.
Email: firsovgm@yandex.ru
ORCID iD: 0000-0002-1262-6532
SPIN-code: 8781-7218
Candidate of Veterinary Sciences, Associate Professor, Department of Veterinary and Sanitary Expertise, Infectious Diseases and Morphology
26 Universitetsky ave., Volgograd, 400002, Russian FederationAleksey A. Ryadnov
Volgograd State Agrarian University
Email: radnov@mail.ru
ORCID iD: 0000-0001-6381-9353
SPIN-code: 3533-9646
Doctor of Biological Sciences, Head of the Department of Veterinary and Sanitary Expertise, Infectious Diseases and Morphology
26 Universitetsky ave., Volgograd, 400002, Russian FederationTamara A. Ryadnova
Volgograd State Agrarian University
Email: radnova@yandex.ru
ORCID iD: 0000-0001-9623-5311
SPIN-code: 5853-4130
Candidate of Biological Sciences, Associate Professor, Department of Veterinary and Sanitary Expertise, Infectious Diseases and Morphology
26 Universitetsky ave., Volgograd, 400002, Russian FederationZoya C. Morozova
Volgograd State Agrarian University
Email: zoyachermen@mail.ru
ORCID iD: 0000-0002-3344-6071
SPIN-code: 3420-5301
Candidate of Biological Sciences, Associate Professor, Department of Veterinary and Sanitary Expertise, Infectious Diseases and Morphology
26 Universitetsky ave., Volgograd, 400002, Russian FederationOleg V. Budtuev
Volgograd State Agrarian University
Email: olegbudtuev@yandex.ru
ORCID iD: 0000-0002-5669-0130
SPIN-code: 2710-4987
Candidate of Agricultural Sciences, Associate Professor of the Department of Veterinary and Sanitary Expertise, Infectious Diseases and Morphology
26 Universitetsky ave., Volgograd, 400002, Russian FederationReferences
- Fredebeul-Krein F, Schmenger A, Wente N, Zhang Y, Krömker V. Factors associated with the severity of clinical mastitis. Pathogens. 2022;11(10):1089. doi: 10.3390/pathogens11101089
- Puerto MA, Shepley E, Cue RI, Warner D, Dubuc J, Vasseur E. The hidden cost of disease: I. Impact of the first incidence of mastitis on production and economic indicators of primiparous dairy cows. Journal of Dairy Science. 2021;104(7):7932–7943. doi: 10.3168/jds.2020-19584
- Ladanova MA, Javadov ED, Plemyashov KV, Stekolnikov AA, Novikova OB. Modern view on the etiology, pathogenesis and diagnosis of mastitis in cows. International Bulletin of Veterinary Medicine. 2021;(4):29–34. (In Russ.). doi: 10.52419/issn2072-2419.2021.4.29
- Ekkhorutomven OT, Medvedev GF. Species composition of microorganisms and their sensitivity to antibiotic drugs in case of mastitis in cows. Animal agriculture and veterinary medicine. 2022;(1):28–33. (In Russ.).
- Lavaee M, Eidi S, Khoramian B. High prevalence of Prototheca spp. and isolation of fungal species in milk samples from cows suffering from mastitis in Mashhad city, northeast Iran. Iranian Journal of Veterinary Science and Technology. 2019;11(2):21–26. doi: 10.22067/veterinary.v11i2.81838
- Kurt S, Eşki F. Pathogen isolation and antibiogram analysis in dairy cows with clinical mastitis in Adana region, Turkey. Etlik Veteriner Mikrobiyoloji Dergisi. 2021;32(1):20–26. doi: 10.35864/evmd.906990
- Firsov GM, Ryadnov AA, Morozova ZC, Ryadnova TA, Firsova YG, Rezyapkina EA, et al. Relationship between the number of somatic cells and pathogens in cow’s milk. Agrarian Russia. 2023;(7):31–35. doi: 10. 30906/1999-5636-2023-7-31-35
- Kanwar R, Aslam MA, Zulqurnain H, Qadeer A, Ali S, Nayab S, et al. Bacteriophages and their endolysin: an alternative therapeutic approach for bovine mastitis. Biol Bull Rev. 2023;13(4):326–335. doi: 10.1134/ S2079086423040059
- Ardicli O, Demirbilek SK, Carli KT. Pathogens isolated from bovine clinical mastitis and their antimicrobial resistance. Medycyna Weterynaryjna. 2022;78(1):19–24. doi: 10.21521/mw.6606
- Rudenko P, Sachivkina N, Vatnikov Y, Shabunin S, Engashev S, Kontsevaya S, et al. Role of microorganisms isolated from cows with mastitis in Moscow region in biofilm formation. Veterinary World. 2021;14(1):40–48. doi: 10.14202/vetworld.2021.40-48
- Morales-Ubaldo AL, Rivero-Perez N, Valladares-Carranza B, Velázquez-Ordoñez V, Delgadillo-Ruiz L, Zaragoza-Bastida A. Bovine mastitis, a worldwide impact disease: Prevalence, antimicrobial resistance, and viable alternative approaches. Veterinary and Animal Science. 2023;21:100306. doi: 10.1016/j.vas.2023.100306
- CLSI. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. 5th ed. Pennsylvania, USA: Clinical and Laboratory Standards Institute; 2018.
- Tommasoni C, Fiore E, Lisuzzo A, Gianesella M. Mastitis in Dairy Cattle: On-Farm Diagnostics and Future Perspectives. Animals. 2023;13(15):2538. doi: 10.3390/ani13152538
- Kornelaeva MV, Karlikova GG. Reproductive capacity and milk production of cows depending on their physio-logical status during lactation. RUDN Journal of Agronomy and Animal Industries. 2022;17(4):484–498. (In Russ.) doi: 10.22363/2312-797X-2022-17-4-484-498
- Wang N, Zhou C, Basang W, Zhu Y, Wang X, Li C, et al. Mechanisms by which mastitis affects reproduction in dairy cows: A review. Reproduction in Domestic Animals. 2021;56(9):1165–1175. doi: 10.1111/rda.13953
- Makavchik SA. Efficiency of definition of Mycoplasma bovis in milk cows with mastitis using a polymerase chain reaction in the real time on a microchip with liophilized systems. International Journal of Veterinary Medicine. 2019;(2):11–16. (In Russ.).
- Rudenko PA, Rudenko AA, Vatnikov YA. Microbial landscape in cows mastitis. Bulletin of the Ulyanovsk State Agricultural Academy. 2020;(2):172–178. (In Russ.). doi: 10.18286/1816-4501-2020-2-172-179
Supplementary files
