RESULTS OF A COMPARATIVE STUDY OF NIGELLA SATIVA L. SEEDS OILS COMPOSITION
- Authors: Goryainov S.V.1, Khromov A.V.1, Bakureza G.1, Cesar E.1, Ivlev V.A.1, Vorobyev A.N.1, Abramovich R.A.1, Potanina O.G.1, Novikov O.O.1
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Affiliations:
- Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
- Issue: Vol 8, No 1 (2020)
- Pages: 29-39
- Section: Articles
- URL: https://journals.rcsi.science/2307-9266/article/view/111627
- DOI: https://doi.org/10.19163/2307-9266-2020-8-1-29-39
- ID: 111627
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Abstract
This article presents results of the chemical composition study of the seeds oils lipid complex of Nigella Sativa L. grown under various geographic conditions. The task of the comprehensive study of the chemical composition of the plant and its individual parts remains relevant due to the wide spectrum of its pharmacological activity.
The aim of this work is a comparative study of the fatty acid composition, a non-saponifiable fraction and the composition of essential oils of Nigella Sativa L. seeds grown in different regions of the world.
Materials and methods. The combination of chromatography-mass spectrometry and 1H-NMR spectroscopy methods made it possible to study the qualitative and quantitative composition of Nigella Sativa L. lipid complex seeds. All the experiments were carried out in accordance with the requirements of the State Pharmacopoeia, 14th Ed, given in the corresponding general pharmacopeial monographs.
Results. Profiles have been established and the content of fatty acids, sterines, triterpene alcohols, essential oils and thymoquinone found out in the lipid complex, has been estimated. The saponifiable portion of the complex is represented by triglycerides (81.7–95.3%), di- (3.9–15.2%) and monoglycerides (0.7–4.1%). They mainly contain linoleic (55.8–60.6%), oleic (21.8–24.6%), palmitic (10.0–12.8%), stearic (2.4–3.2 %) and cis-11.14-eicosadiene (2.3–2.6%) acids. In the lipid complex, the contents of sterines and triterpene alcohols were 0.4–0.7%; up to 70% of the fraction was represented by β-sitosterol (22.5–29.2%), cycloartenol (20.1–36.6%) and 24 methylenecycloartanol (9.5–19.9%). In the trace amounts (up to 1.0%), cholesterol has been detected in all the samples. In the lipid complexes, the content of thymoquinone ranged from 0.7 to 2.6%.
Conclusion. A comparative study of the seeds lipid complex of Nigella Sativa L. grown under various geographic conditions, has been carried out. The marker compounds as well as their content standards for determining the authenticity of raw materials (thymoquinone, para-cimen, cis-11.14-eicosadienic acid), have been identified.
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##article.viewOnOriginalSite##About the authors
Sergey V. Goryainov
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: goryainovs@list.ru
ORCID iD: 0000-0002-7625-9110
head of the laboratory for high-resolution mass spectrometry and NMR spectroscopy of the Center for Precision Instrumental Methods of Analysis (PRIMA), the Shared Research and Education Center (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowArkady V. Khromov
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: arkadiy18@ya.ru
ORCID iD: 0000-0002-6355-5615
Candidate of Technical Sciences, Director of the Center for Social Security and Safety, the Shared Research and Education Center (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowGohara Bakureza
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: gwohara2016@gmail.com
post-graduate student, Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowEsparsa Cesar
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: cesaug@yandex.ru
ORCID iD: 0000-0002-8200-6208
Candidate of Sciences (Chemistry), Engineer of the Laboratory for High Resolution Mass Spectrometry and NMR Spectroscopy of the Center for Precision Instrumental Analysis Methods (PRIMA), the Shared Research and Education Center (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowVasily A. Ivlev
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: chemistron@mail.ru
ORCID iD: 0000-0001-9664-9506
Engineer of the Laboratory for High Resolution Mass Spectrometry and NMR Spectroscopy of the Center for Precision Instrumental Analysis Methods (PRIMA), the Shared Research and Education Center (REC), (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowAlexander N. Vorobyev
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: alek_san2007@mail.ru
ORCID iD: 0000-0002-7182-9911
Candidate of Sciences (Pharmacy), Head of the Laboratory of Industrial Pharmaceutical Technology, the Center for Precision Instrumental Analysis Methods (PRIMA), the Shared Research and Education Center (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowRimma A. Abramovich
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: abr-rimma@yandex.ru
ORCID iD: 0000-0003-1784-881X
Doctor of Sciences (Pharmacy), Associate Professor, Director of the Shared Research and Education Center, Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowOlga G. Potanina
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Email: microly@mail.ru
ORCID iD: 0000-0002-0284-419X
Doctor of Sciences (Pharmacy), Head of the Department of Pharmaceutical Chemistry and Pharmacognosy, the Shared Research and Education Center (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowOleg O. Novikov
Federal State Autonomous Educational Institution for Higher Education “Peoples’ Friendship University of Russia”
Author for correspondence.
Email: novikov_oo@rudn.university
ORCID iD: 0000-0003-3145-6783
Doctor of Sciences (Pharmacy), Professor, Director of CLKKLSIMI, the Shared Research and Education Center (REC), Federal State Autonomous Educational Institution of Higher Education, Peoples’ Friendship University of Russia.
Russian Federation, MoscowReferences
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