DETERMINATION OF RUTIN IN LEAVES OF SALIx TRIANDRA USING THE METHOD OF PLANAR CHROMATOGRAPHY
- Authors: Sannikova E.G1, Mezenova T.D1
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Affiliations:
- Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University of the Ministry of Health of Russia
- Issue: Vol 4, No 1 (2016)
- Pages: 85-90
- Section: Articles
- URL: https://journals.rcsi.science/2307-9266/article/view/111460
- DOI: https://doi.org/10.19163/2307-9266-2016-4-1(14)-85-90
- ID: 111460
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Abstract
The aim of the study is to develop new methods of identification and quantitative determination of rutin in the leaves of Salix triandra L. with thin-layer chromatography. The digital processing of the chromatograms was performed using «Sorbfil Videodensitometer» computer program (Krasnodar). For the chromatography analysis we used plates «Sorbfil» PTSH-P-A-UV, mobile phase: n-butanol-glacial acetic acid-water (4:1:1), detecting reagent - ammonia vapors. The rutin is detected as yellow spots with Rf 0.64±0.02. The detection limit is 0.5 µg/µl. The regression equation has the form: S=4.75×103 m. With the use of this method we found that the composition of rutin in leaves of Salix triandra was in the range 1.81±0.06% on the air-dry raw material (relative standard deviation RSD%=2.5%; the measurement error E=3.12%). The method is sensitive, linear, has a small error, and does not require expensive equipment.
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##article.viewOnOriginalSite##About the authors
E. G Sannikova
Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University of the Ministry of Health of RussiaPyatigorsk
T. D Mezenova
Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University of the Ministry of Health of Russia
Email: mezenova@yandex.ru
Pyatigorsk
References
- Государственная фармакопея СССР. - ХI изд. - М.: Медицина, 1987. - Вып. 1. - С. 199-251.
- Компанцев В.А. Химическое изучение фенольных гликозидов некоторых видов ив Северного Кавказа: дис. … канд. фармацевт. наук. - Пятигорск, 1970. - 163 с.
- Род 356. Ива - Salix L. Флора СССР: в 30 т. // Под ред. акад. В.Л. Комарова. - М. - Л.: Изд-во АН СССР, 1936. - Т. V. - С. 184-186.