Phenotypic and molecular genetic analysis of Brucella spp. strains isolated from atypical carriers
- Authors: Serdyuk N.S.1, Zharinova N.V.1, Zhilchenko E.B.1, Karapetyan M.G.1, Belozerova O.N.1
-
Affiliations:
- Stavropol Anti-Plague Institute
- Issue: Vol 15, No 2 (2025)
- Pages: 80-84
- Section: Original Investigations
- URL: https://journals.rcsi.science/2226-6976/article/view/314506
- DOI: https://doi.org/10.18565/epidem.2025.15.2.80-84
- ID: 314506
Cite item
Open Access
Access granted
Subscription Access
Abstract
Objective. Evaluation of the phenotypic and genetic properties of Brucella spp. isolated in the Russian Federation from different types of carriers.
Materials and methods. We used 38 strains of the causative agent of brucellosis from the collection of the Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being. The phenotypic properties of the cultures were studied using generally accepted phenotypic methods. Whole genome sequencing of DNA of 38 isolates was performed using Ion PGM (Life Technologies, USA).
Results. The cultures had typical tinctorial, morphological and cultural properties characteristic of B. melitensis, B. abortus, B. suis. As a result of the comprehensive study, it was established that the studied strains of the causative agent of brucellosis (B. melitensis, B. abortus and B. suis), isolated in the territory of the Russian Federation from atypical hosts, do not differ in their cultural-morphological, molecular-genetic properties from the cultures isolated from the main carriers of the infection.
Conclusion. Brucella strains retain their basic properties when passing from the main hosts to atypical carriers.
Full Text
##article.viewOnOriginalSite##About the authors
Natalia S. Serdyuk
Stavropol Anti-Plague Institute
Email: nata.serduk.83@mail.ru
ORCID iD: 0000-0001-5350-0111
Сand. Biol. Sci., Senior Researcher at the Collection of Pathogenic Microorganisms Laboratory
Russian Federation, StavropolNina V. Zharinova
Stavropol Anti-Plague Institute
Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-6718-4877
Сand. Biol. Sci., Senior Researcher at the Collection of Pathogenic Microorganisms Laboratory
Russian Federation, StavropolElena B. Zhilchenko
Stavropol Anti-Plague Institute
Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-7180-1292
Сand. Biol. Sci., Head of the Collection of Pathogenic Microorganisms Laboratory
Russian Federation, StavropolManya G. Karapetyan
Stavropol Anti-Plague Institute
Email: stavnipchi@mail.ru
ORCID iD: 0009-0005-3006-5504
Junior Researcher at the Collection of Pathogenic Microorganisms Laboratory
Russian Federation, StavropolOlga N. Belozerova
Stavropol Anti-Plague Institute
Author for correspondence.
Email: stavnipchi@mail.ru
ORCID iD: 0009-0002-5570-557X
Junior Researcher at the Collection of Pathogenic Microorganisms Laboratory
Russian Federation, StavropolReferences
- Нургаянова Г.А., Белоусов В.И., Разумова А.А., Шарыпов А.С., Черных О.Ю., Малов Д.Н. Бруцеллез животных. Монография. М., 2022. 340 с. doi: 10.18720/SPBPU/2/z22-3. Nurgayanova G.A., Belousov V.I., Razumova A.A., Sharypov A.S., Chernykh O.Yu., Malov D.N. Animal brucellosis. Monograph. M., 2022. 340 p. (In Russ.) doi: 10.18720/SPBPU/2/z22-3
- Корошенко В.А., Щипелева И.А., Кретенчук О.Ф., Марковская Е.И. Прошлое, настоящее, перспективы и проблемы совершенствования специфической профилактики бруцеллеза. Медицинский вестник Юга России 2021; (3): 12–21. doi: 10.21886/2219-8075-2021-12-3-12-21. Koroshenko V.A., Shchipeleva I.A., Kretenchuk O.F., Markovskaya E.I. The past, present, prospects and problems of improving the specific prevention of brucellosis. Medical Herald of the South of Russia 2021; (3): 12–21. (In Russ.). doi: 10.21886/2219-8075-2021-12-3-12-21
- Пономаренко Д.Г., Скударева О.Н., Хачатурова А.А., Лукашевич Д.Е., Жаринова И.В., Даурова А.В. и др. Бруцеллез: тенденции развития ситуации в мире и прогноз на 2022 г. в Российской Федерации. Проблемы особо опасных инфекций 2022; (2): 35–45. doi: 10.21055/0370-1069-2022-2-36-45. Ponomarenko D.G., Skudareva O.N., Khachaturova A.A., Lukashevich D.E., Zharinova I.V., Daurova A.V. et al. Brucellosis: trends in the development of the situation in the world and forecast for 2022 in the Russian Federation. Problems of especially dangerous infections 2022; (2): 35–45. (In Russ.) doi: 10.21055/0370-1069-2022-2-36-45
- Насибуллин Р.Ю., Тухватуллина Л.А., Богова Я.А., Сафина Г.М., Косарев М.А. Бруцеллез: его распространение и профилактика. Ветеринарный врач 2021; (1): 38–43. doi: 10.33632/1998-698Х.2021-1-38-43. Nasibullin R.Yu., Tukhvatullina L.A., Bogova Y.A., Safina G.M., Kosarev M.A. Brucellosis: its spread and prevention. Veterinarian 2021; (1): 38–43. (In Russ.) doi: 10.33632/1998-698Х.2021-1-38-43
- Сердюк Н.С., Жилченко Е.Б., Жаринова Н.В., Царева Н.С., Ковалев Д.А., Писаренко С.В. Анализ штаммов Brucella melitensis, выделенных на территории Республики Бурятия от нетипичных бруцеллоносителей. Проблемы особо опасных инфекций на Северном Кавказе: Материалы региональной науч.-практ. конф. с международным участием, посвященной 70-летию со дня основания ФКУЗ Ставропольский противочумный институт Роспотребнадзора. Ставрополь: ООО «Экспо-Медиа», 2022: 185–186. Serdyuk N.S., Zhilchenko E.B., Zharinova N.V., Tsareva N.S., Kovalev D.A., Pisarenko S.V. Analysis of Brucella melitensis strains isolated in the Republic of Buryatia from atypical brucellosis carriers. Problems of especially dangerous infections in the North Caucasus: Proceedings of the regional scientific and practical conference with international participation, dedicated to the 70th anniversary of the foundation of the Stavropol Anti-Plague Institute of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, Stavropol: Expo-Media, 2022: 185–186. (In Russ.)
- Нулыгаянова Г.А., Белоусов В.И., Варенцова А.А., Шарыпов А.С., Шубина Е. А., Скляров О.Д. и др. Диагностика бруцеллеза животных в Российской Федерации. Ветеринария сельскохозяйственных животных 2021; (8): 3–9. Nulygayanova G.A., Belousov V.I., Varentsova A.A., Sharypov A.S., Shubina E.A., Sklyarov O.D. et al. Diagnostics of animal brucellosis in the Russian Federation. Veterinary Science of Farm Animals 2021; (8): 3–9. (In Russ.).
- Schwengers O., Jelonek L., Dieckmann M.A., Beyvers S., Blom J., Goesmann A. Bakta: rapid and standardized annotation of bacterial genomes via alignment-free sequence identification. Microb. Genom. 2021; 7(11): 13–17. doi: 10.1099/mgen.0.000685
- Letunic I., Bork P. Interactive Tree of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic Acids Res. 2021; 49(W1): W293–W296. doi: 10.1093/nar/gkab301
- Кузнецова И.В., Ковалев Д.А., Писаренко С.В., Бобрышева О.В., Шапаков Н.А., Жиров А.М. и др. Генетическая характеристика штаммов Brucella melitensis, выделенных на территории Российской Федерации, на основе данных анализа единичных нуклеотидных полиморфизмов при полногеномном секвенировании. Проблемы особо опасных инфекций. 2024; (1): 154–161. doi: 10.21055/0370-1069-2024-1-154-161. Kuznetsova I.V., Kovalev D.A., Pisarenko S.V., Bobrysheva O.V., Shapakov N.A., Zhirov A.M. et al. Genetic characteristics of Brucella melitensis strains isolated in the Russian Federation based on single nucleotide polymorphism analysis data from whole-genome sequencing. Problems of Particularly Dangerous Infections. 2024; 1: 154–161. (In Russ.) doi: 10.21055/0370-1069-2024-1-154-161
- Ковалев Д.А., Шапаков Н.А., Писаренко С.В., Бобрышева О.В., Пономаренко Д.Г., Хачатурова А.А., Куличенко А.Н. Филогеномный анализ штаммов Brucella abortus, выделенных на территории Российской Федерации. Бактериология 2024; 9(4): 107–114. doi: 10.20953/2500-1027-2024-4-107-114. Kovalev D.A., Shapakov N.A., Pisarenko S.V., Bobrysheva O.V., Ponomarenko D.G., Khachaturova A.A., Kulichenko A.N. Phylogenomic analysis of Brucella abortus strains isolated in the Russian Federation. Bacteriology 2024; 9(4): 107–114. (In Russ.) doi: 10.20953/2500-1027-2024-4-107-114
Supplementary files
