Diagnostic value of chaperone activity of heat shock proteins 70 in coronary atherosclerosis


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Abstract

Aim. The aim of the research was to study the role of chaperone activity heat shock proteins (HSP) 70 in pathogenesis and diagnostic in patients with coronary atherosclerosis. Materials and methods. We examined 354 patients with coronary heart disease, who had coronary atherosclerosis of varying degrees, according to coronary angiography (was performed by the Judkins technique). The severity of coronary atherosclerosis was determined on the basis of the Gensini index. According to the Gensini index, patients were divided into 2 groups: GS0 - 152 patients without signs of coronary atherosclerosis, GS1 - 202 patients with coronary lesions. Chaperone activity was determined by thermodynamic method. Results. The study showed significant differences in the level of chaperone activity HSP70 in patients with different severity of coronary atherosclerosis. The correlation analysis revealed a significant negative relationship between chaperone activity HSP70 and the Gensini index. The cut-off value of chaperone activity of HSP70, by which can be judged on the presence or absence of coronary atherosclerosis, is establish. Conclusion. The revealed threshold of chaperone activity can be considered as a possible marker of the severity of coronary atherosclerosis.

About the authors

Julia A. Kotova

Burdenko Voronezh State Medical University

Email: kotova_u@inbox.ru
канд. мед. наук, доц. каф. поликлинической терапии Voronezh, Russia

Anna A. Zuikova

Burdenko Voronezh State Medical University

D. Sci. (Med.), Prof. Voronezh, Russia

Alexander N. Pashkov

Burdenko Voronezh State Medical University

D. Sci. (Biol.), Prof. Voronezh, Russia

References

  1. Рагино Ю.И., Чернявский А.М., Еременко Н.В. и др. Ключевые лабораторно-диагностические биомаркеры коронарного атеросклероза. Кардиология. 2011; 3: 42-6. @@Ragino Iu.I., Cherniavskii A.M., Eremenko N.V. et al. Kliuchevye laboratorno-diagnosticheskie biomarkery koronarnogo ateroskleroza. Kardiologiia. 2011; 3: 42-6 (in Russian).]
  2. Рудакова Д.М., Веселовская Н.Г., Чумакова Г.А. Коронарный атеросклероз у мужчин с метаболическим синдромом: факторы риска, роль миокардиальных цитопротекторов. Доктор.ру. 2017; 10 (139): 15-20. @@Rudakova D.M., Veselovskaia N.G., Chumakova G.A. Koronarnyi ateroskleroz u muzhchin s metabolicheskim sindromom: faktory riska, rol' miokardial'nykh tsitoprotektorov. Doktor.ru. 2017; 10 (139): 15-20 (in Russian).]
  3. Negre-Salvavre A, Auge N, Camare C et al. Dual signaling evoked by oxidized LDLs in vascular cells. Free Radic Biol Med 2017; 106: 118-33.
  4. Wick G, Knoflach M, Xu Q. Autoimmune and inflammatory mechanisms in atherosclerosis. Annu Rev Immunol 2017; 22: 361-403.
  5. Пастухов Ю.Ф., Екимова И.В. Молекулярные, клеточные и системные механизмы протективной функции белка теплового шока 70 кДа. Нейронауки. 2005; 2 (2): 3-25. @@Pastukhov Iu.F., Ekimova I.V. Molekuliarnye, kletochnye i sistemnye mekhanizmy protektivnoi funktsii belka teplovogo shoka 70 kDa. Neironauki. 2005; 2 (2): 3-25 (in Russian).]
  6. Martin-Ventura J.L, Leclerq A, Blanco-Colio LM et al. Low plasma levels of HSP70 in patients with carotid atherosclerosis are associated with increased levels of proteolytic markers of neutrophil activation. Atherosclerosis 2007; 194: 334-41.
  7. Xie F, Zhan R, Yan L.C et al. Diet-induced elevation of circulating HSP70 may trigger cell adhesion and promote the development of atherosclerosis in rats. Cell Stress Chaperones 2016; 21: 907-14.
  8. Bielecka-Dabrowa A. HSP 70 and atherosclerosis-protector or activator? Expert Opin Ther Targets 2009: 13: 307-17.
  9. Gensini Goffredo G. A more meaningful scoring system for determining the severity of coronary artery disease. Am J Cardiol 1983; 51: 606. doi: 10.1016/S0002-9149(83)80105-2
  10. Борзова В.А. Механизмы защитного действия шаперонов при агрегации белков. Дис.. канд. биол. наук. М., 2016. @@Borzova V.A. Mekhanizmy zashchitnogo deistviia shaperonov pri agregatsii belkov. Dis.. kand. biol. nauk. Moscow, 2016 (in Russian)
  11. Котова Ю.А., Зуйкова А.А., Пашков А.Н. и др. Шаперонная активность в диагностике коронарного атеросклероза. Системный анализ и управление в биомедицинских системах. 2018; 17 (3): 563-6. @@Kotova Iu.A., Zuikova A.A., Pashkov A.N. et al. Shaperonnaia aktivnost' v diagnostike koronarnogo ateroskleroza. Sistemnyi analiz i upravlenie v biomeditsinskikh sistemakh. 2018; 17 (3): 563-6 (in Russian).]
  12. Morimoto R.I, Kline M.P, Bimston D.N, Cotto J.J. The heat-shock response: regulation and function of heat-shock proteins and molecular shaperones. Essays biochem 1997; 32: 17-29.
  13. Willis M.W, Patterson C. Hold me tight: the role of HSP family of chaperones in cardiac desease. Circulation 2010; 122 (17): 1740-51.
  14. Dulin E, García-Barreno P, Guisasola M.C. Extracellular heat shock protein 70 (HSPA1A) and classical vascular risk factors in a general population. Cell Stress Chaperones 2010; 15 (6): 929-37.

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