Morphometric analysis of the intercellular substance of hypertrophic scars after anti-scar treatment
- Authors: Minaev S.V.1, Grigorova A.N.1, Vladimirova O.V.1,2, Ivchenko A.A.1, Lavreshin P.M.1,2, Vladimirov V.I.2, Timofeev S.I.3,4, Gobedzhishvili V.V.5
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Affiliations:
- Stavropol State Medical University
- City Clinical Hospital No. 2
- Magadan Regional Children’s Hospital
- Far Eastern State Medical University
- I.M. Sechenov First Moscow State Medical University (Sechenov University)
- Issue: Vol 11, No 1 (2021)
- Pages: 39-46
- Section: Original Study Articles
- URL: https://journals.rcsi.science/2219-4061/article/view/123519
- DOI: https://doi.org/10.17816/psaic711
- ID: 123519
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Abstract
AIM: This study aimed to assess the formation of scar tissue after burns under the influence of an anti-scar gel. Understanding of the processes involving scars and the morphofunctional features of the tissue at different stages of development allows targeted selection of therapy and prevention of scars.
MATERIALS AND METHODS: We conducted a comparative prospective analysis from 2005 to 2020. Of which two groups were identified. In group 1 (n = 47), burns were treated according to the standard scheme without the use of modern wound coverings. In group 2 (n = 41), early primary prevention of pathological scarring was performed, where the Contractubex gel was applied to the area of burn injury from the moment of epithelialization. Histological examination included the analysis of skin biopsies in the area of damage before and after conservative treatment.
RESULTS: Histological examination showed quantitative changes in the cellular composition of the scar tissue in all groups. The average quantitative index of the fibroblast activity was significantly reduced in group 2 using Contractubex gel. Thickness of collagen fibers, according to the morphometric analysis, is most reduced in all layers of the dermis in group 2 (p < 0.05). In group 1, collagen fibers are represented as nodular clusters; in some areas of the reticular layer of the dermis, fibers have a more fragmented appearance. In group 2, the use of Contractubex leads to a significant decrease in the level of tumor growth factor-β in the papillary and reticular layers of the dermis.
CONCLUSION: The use of Contractubex gel in the early prevention of pathological scarring significantly reduces the need for subsequent reconstructive surgical interventions
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##article.viewOnOriginalSite##About the authors
Sergey V. Minaev
Stavropol State Medical University
Email: sminaev@yandex.ru
ORCID iD: 0000-0002-8405-6022
SPIN-code: 3113-6982
Dr. Sci. (Med.), Professor
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Alina N. Grigorova
Stavropol State Medical University
Author for correspondence.
Email: alina.mashchenko@mail.ru
ORCID iD: 0000-0001-5020-232X
SPIN-code: 1762-8310
https://www.facebook.com/profile.php?id=100016382449091
Cand. Sci. (Med.), Associate Professor
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Oksana V. Vladimirova
Stavropol State Medical University; City Clinical Hospital No. 2
Email: oxy_8181@mail.ru
ORCID iD: 0000-0002-3011-7408
SPIN-code: 2162-1432
Cand. Sci. (Med.)
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Anna A. Ivchenko
Stavropol State Medical University
Email: ivchenkoanna1978@gmail.com
SPIN-code: 7729-3545
Assistant of the Department
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Petr M. Lavreshin
Stavropol State Medical University; City Clinical Hospital No. 2
Email: v_lin@mail.ru
ORCID iD: 0000-0002-7375-8950
SPIN-code: 4862-3480
Dr. Sci. (Med.), Professor
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Vladimir I. Vladimirov
City Clinical Hospital No. 2
Email: vla3788@yandex.ru
SPIN-code: 3520-0968
MD, PhD, surgery, oncologist
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Sergey Ivanovich I. Timofeev
Magadan Regional Children’s Hospital; Far Eastern State Medical University
Email: timofeev_si@mail.ru
ORCID iD: 0000-0002-5808-0686
SPIN-code: 5457-1995
Cand. Sci. (Med.)
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500Vakhtang V. Gobedzhishvili
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: walker87@list.ru
ORCID iD: 0000-0002-8883-4952
SPIN-code: 3486-4344
Cand. Sci. (Med.)
Russian Federation, 5 Rogozhnikova str., Stavropol, 35500References
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