Email this article The results of genotyping in HPV-associated cervical infection
- Authors: Klinyshkova TV1, Buyan MS1
-
Affiliations:
- Omsk State Medical University of the Ministry of Health of the Russian Federation
- Issue: Vol 18, No 3 (2016)
- Pages: 16-19
- Section: Articles
- URL: https://journals.rcsi.science/2079-5831/article/view/28525
- ID: 28525
Cite item
Abstract
Aim: to evaluate the quantitative and qualitative composition of cervical human papillomavirus (HPV) infection in chronic cervicitis (CC). Materials and methods. The study included 102 patients with cervical HPV infection, mean age 33,9±1,1 years. The 1st group (main) consisted of patients with CC (n=39), 2nd group (comparison) - the patients with cervical intraepithelial neoplasia - CIN (n=50), group 3 (comparison) - patients with cervical cancer (n=13). Complex diag-cer nostics included liquid cytology, colposcopic, and histological examination of cervical biopsies and endocervical samples, advanced screening for high risk HPV DNA (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 types) and low risk (6, 11 types) with viral load detection, screening for chlamydia and mycoplasmas DNA. Statistical analysis of data was performed using STATISTICA-6, functionality of MS Excel. Results. The prevalence of the same HPV type (56% and 64% respectively), two types (26 and 22%), three or more types (18 and 14%) in the 1st and 2nd group had no significant difference respectively. In the 1st group 45, 52 relative prevalence of HPV types was found in comparison with CIN, in which the predominance of 33 and 35 types of HPV (p<0.05) was marked. First group patients are characterized by the more frequent detection of clinically insignificant (<3 lg×105) level of high risk HPV viral load (49%) compared with CIN (32%) at which threshold progression exceeding (>5 lg×105) was observed significantly more frequently (p<0.05). Conclusion. Among patients with СC an absence of differences in mono - and combined HPV infection prevalence, in detection rate of 16, 18, 31, 51, 56, >58, and 59 HPV types was shown in comparison with CIN, except for the viral load level.
Keywords
Full Text
##article.viewOnOriginalSite##About the authors
T V Klinyshkova
Omsk State Medical University of the Ministry of Health of the Russian Federation
Email: klin_tatyana@mail.ru
д-р мед. наук, проф. каф. акушерства и гинекологии ПДО ГБОУ ВПО ОмГМУ 644099, Russian Federation, Omsk, ul. Lenina, d. 12
M S Buyan
Omsk State Medical University of the Ministry of Health of the Russian Federation
Email: mam_agata@mail.ru
соискатель каф. акушерства и гинекологии ПДО ГБОУ ВПО ОмГМУ 644099, Russian Federation, Omsk, ul. Lenina, d. 12
References
- Прилепская В.Н., Довлетханова Э.Р. Папилломавирусная инфекция - от ранней диагностики к рациональной терапии. Акуш. и гинекол. 2013; 2: 101-7.
- Tota J.E, Chevarie-Davis M, Richardson L.A et al. Epidemiology and burden of HPV infection and related diseases: implications for prevention strategies. Prev Med 2011; 53 (1): 12-21.
- Клинышкова Т.В., Каратюк Т.И., Турчанинов Д.В. Факторы риска развития предрака шейки матки. Врач. 2012; 11: 56-8.
- Унанян А.Л. Коссович Ю.М. Хронический цервицит и хронический эндометрит: общие аспекты патогенеза и патогенетической терапии. Рос. вестн. акушера - гинеколога. 2012; 6: 40-5.
- Мальцева Л.И., Фаррахова Л.Н., Ахметзянова А.В., Нигматуллина Н.А. Оценка ВПЧ-ассоциированного хронического цервицита, как фактора риска развития рака шейки матки. Практ. медицина. 2012; 9: 73-6.
- Shen X.H, Liu S.H. Human papillomavirus genotypes associated with mucopurulent cervicitis and cervical cancer in Hangzhou, China. Asian Pac J Cancer Prev 2013; 14 (6): 3603-6.
- Ding X, Liu Z, Su J et al. Human papillomavirus type - specific prevalence in women referred for colposcopic examination in Beijing. J Med Virol 2014; 86 (11): 1937-43.
- Li S.M, Zhang W.H, Wu L.Y et al. Preliminary study on the relationship between loads of human papillomavirus in cervical carcinoma and cervical intraepithelial neoplasia. Zhonghua Fu Chan Ke Za Zhi 2004; 39 (6): 400-2.
- Nwachokor F.N, Forae G.C. Morphological spectrum of non - neoplastic lesions of the uterine cervix in Warri, South-South, Nigeria. Niger J Clin Pract 2013; 16: 429-32.
- Абрамовских О.С., Алехина К.А., Савочкина А.Ю., Пряхина К.Е. Микробный пейзаж репродуктивного тракта женщин с папилломавирусной инфекцией. Вестн. Челябинского государственного университета. 2013; 7; 46-7.
- Качалина Т.С., Шахова Н.М., Качалина О.В., Елисеева Д.Д. Хронический цервицит и ВПЧ инфекция в репродуктивном возрасте. Пути снижения диагностической и лечебной агрессии. Акушерство, гинекология и репродукция. 2012; 4: 6-12.
- Куевда Д.А., Шипулина О.Ю., Минкина Г.Н., Пиксасова О.В. Современные требования к диагностике генитальной папилломавирусной инфекции: количественный подход. Тезисы 2-го Всероссийского конгресса дерматовенерологов. СПб., 2007.
- Shen G, Cheng J, Wang Y et al. Viral DNA load of high - risk human papilloma virus is closely associated with the grade of cervical lesions. Zhou P1. Int J Clin Exp Med 2014; 7 (12): 5826-31.
- Пестрикова Т.Ю., Панфилова Ю.О. Чувствительность патогенной микрофлоры цервикального канала к антибактериальным препаратам у пациенток с хроническим цервицитом и вирусом папилломы человека//Гинекология. 2015; 17 (1): 18-21.
- Апгар Б.С., Броцман Г.Л., Шпицер М. Клиническая кольпоскопия. Практическое руководство. Под ред. В.Н.Прилепской, Т.Н.Бебневой. М.: Практическая медицина, 2014.
- Свердлова Е.С., Дианова Т.В., Кменщикова Н.В. Вирусные заболевания шейки матки. Сиб. мед. журн. 2011; 6: 135-7.
- Feng Y.K, Peng Y, Zhu L, Niu X.Y. Relationship of Human Papillomavirus Subtypes and Multiple Infection with Different Cervical Precancerous Diseases in Sichuan Province. Sichuan Da Xue Xue Bao Yi Xue Ban 2015; 46 (3): 422-5.
- Rahman T, Tabassum S, Jahan M, Nessa A et al. Detection and estimation of human papillomavirus viral load in patients with cervical lesions. Bangladesh Med Res Counc Bull 2013; 39 (2): 86-90.
Supplementary files
