Non-invasive fetal RHD determination in out-patient practice


Cite item

Full Text

Abstract

The aim of the study was to improve approaches to the use of Rh0(D) immune globulin in pregnant women with Rh-negative blood by introducing noninvasive method of determination of fetal rhesus blood factor by mother’s blood into curation program of pregnancy at risk of RBC sensitization. The accuracy of noninvasive method of rhesus genotyping in 204 pregnant women with Rh negative blood in 14-27 weeks of pregnancy was 99.0%. The determination of fetal rhesus allows personify implementation of immunological prophylaxis in Rh negative pregnant women.

About the authors

S M Makhmudova

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation; City clinical hospital №195 of the Department of Health of Moscow

врач акушер-гинеколог ГБУЗ ГП №195, НЦАГиП им. акад. В.И.Кулакова 121614, Russian Federation, Moscow, ul. Krylatskie kholmy, d. 51

S V Pavlovich

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation

Email: st.pavlovich@mail.ru
ученый секретарь ФГБУ НЦАГиП им. акад. В.И.Кулакова 117997, Russian Federation, Moscow, ul. Akademika Oparina, d. 4

D Iu Trofimov

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation

зав. лаб. молекулярно-генетических методов ФГБУ НЦАГиП им. акад. В.И.Кулакова 117997, Russian Federation, Moscow, ul. Akademika Oparina, d. 4

A E Donnikov

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation

ст. науч. сотр. лаб. молекулярно-генетических методов ФГБУ НЦАГиП им. акад. В.И.Кулакова 117997, Russian Federation, Moscow, ul. Akademika Oparina, d. 4

L Z Faizulin

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation

вед. науч. сотр. лаб. молекулярно-генетических методов ФГБУ НЦАГиП им. акад. В.И.Кулакова 117997, Russian Federation, Moscow, ul. Akademika Oparina, d. 4

A A D'iakonova

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation

ст. науч. сотр. 2-го отд-ния акушерского патологии беременности ФГБУ НЦАГиП им. акад. В.И.Кулакова 117997, Russian Federation, Moscow, ul. Akademika Oparina, d. 4

V N Karnaukhov

V.I.Kulakov Research Center for Obstetrics, Gynecology and Perinatology of the Ministry of Health of the Russian Federation

мл. науч. сотр. лаб. молекулярно-генетических методов ФГБУ НЦАГиП им. акад. В.И.Кулакова 117997, Russian Federation, Moscow, ul. Akademika Oparina, d. 4

References

  1. Mollison P.L, Engelfriet C.P, Contreras M. Blood Transfusion in Clinical Medicine, 10th ed. Oxford, Blackwell Science Ltd., 1997.
  2. Lo Y.M, Hjelm N.M, Fidler C et al. Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma. N Engl J Med 1998; 339: 1734-8.
  3. Clausen F.B, Krog G.R, Rieneck K et al. Reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD negative women. Prenat Diagn 2005; 25: 1040-4.
  4. Engelfriet C.P, Reesink H.W, Judd W.J et al. Current status of immunoprophylaxis with anti-D immunoglobin.Vox Sang 2003; 85: 328-37.
  5. Flegel W.A, Wagner F.F. Molecular genetics of RH. Vox Sang 2000; 78 (Suppl. 2): 109-15.
  6. Mackenzie I.Z, Roseman F, Findlay J et al. The kinetics of routine antenatal prophylactic intramuscular injections of polyclonal anti-D immunoglobulin. BJOG 2006; 113: 97-101.
  7. Daniels G. Human Blood Groups, 2nd ed. Oxford, Blackwell Science Ltd., 2002.
  8. Van der Schoot C.E, Soussan A.A, Koelewijn J et al. Noninvasive antenatal RHD typing. Transfus Clin Biol 2006; 13: 53-7.
  9. Тетруашвили Н.К., Федорова Н.И., Файзуллин Л.З., Карнаухов В.Н. Определение свободной эмбриональной ДНК в плазме крови беременных для неинвазивной пренатальной генетической диагностики. Акуш. и гинекол. 2012; 6: 4-8.
  10. Finning K.M, Martin P.G, Soothill P.W, Avent N.D. Prediction of fetal D status from maternal plasma: introduction of a new noninvasive fetal RHD genotyping service. Transfus 2002; 42 (8): 1079-85.
  11. Rouillac-Le Sciellour C, Puillandre P, Gillot R et al. Largescale pre - diagnosis study of fetal RHD genotyping by PCR on plasma DNA from RhD-negative pregnant women. Mol Diagn 2004; 8 (1): 23-31.
  12. Minon J.M, Gerard C, Senterre J.M et al. Routine fetal RHD genotyping with maternal plasma: a four - year experience in Belgium. Transfus 2008; 48 (2): 373-81.
  13. Hromadnikova I, Vechetova L, Vesela K et al. Non - invasive fetal RHD exon 7 and exon 10 genotyping using real - time PCR testing of fetal DNA in maternal plasma. Fetal Diagn Ther 2005; 20 (4): 275-80.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2016 Consilium Medicum

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Согласие на обработку персональных данных

 

Используя сайт https://journals.rcsi.science, я (далее – «Пользователь» или «Субъект персональных данных») даю согласие на обработку персональных данных на этом сайте (текст Согласия) и на обработку персональных данных с помощью сервиса «Яндекс.Метрика» (текст Согласия).