电邮这篇文章 Uterine microbiome in women with uterine scar defect after cesarean section: Prospective cohort study
- 作者: Michelson A.A.1, Telyakova M.I.1, Lazukina M.V.1, Chistyakova G.N.1, Ustyuzhanin A.V.1, Varaksin A.N.2, Maslakova T.A.2, Konstantinova E.D.2
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隶属关系:
- Urals Scientific Research Institute for Maternal and Child Care
- Institute of Industrial Ecology
- 期: 卷 25, 编号 3 (2023)
- 页面: 348-352
- 栏目: ORIGINAL ARTICLE
- URL: https://journals.rcsi.science/2079-5831/article/view/253869
- DOI: https://doi.org/10.26442/20795696.2023.3.202353
- ID: 253869
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Aim. To evaluate the endometrial microbiota in patients with uterine scar defect after cesarean section.
Materials and methods. The study included 80 women of reproductive age. The main group included 50 patients with a uterus scar defect due to cesarean section; the comparison group included 30 women with a competent uterus scar due to cesarean section. Patients underwent a Pipelle endometrial biopsy on days 20–22 of the menstrual cycle using a double-lumen catheter that excludes sample contamination with vaginal and cervical microflora. A molecular genetic study of the endometrium was performed by real-time polymerase chain reaction using the Femoflor 16 reagent kit (DNA-Technology
Moscow). The DNA content in the specimen was measured using software and expressed in genome equivalent (GE) proportional to the number of microorganisms. The statistical data were processed using the Exсel software package and SPSS Statistics 22.0.
Results. The study showed the following statistically significant differences: lower count of Lactobacillus spp. – 2.600 (1.430–3.600) GE/mL vs 3.550 (2.800–4.700) GE/mL in patients of the comparison group (p=0.02); higher count of Streptococcus and Staphylococcus spp. – 3.270 (3.000–3.700) GE/mL and 3.450 (3.200–3.600) GE/mL vs 1.030 (0.760–1.700) GE/mL and 0.560 (0.120–1.200) GE/mL in the comparison group, respectively (p<0.00001); higher count of Enterobacteriaceae – 2.700 (1.700–3.300) GE/mL vs 0.950 (0.660–1.120) GE/mL in the comparison group (p<0.00001); higher count of Gardnerella/Prevotellabivia/Porphyromonas spp. – 2.310 (0.930–3.480) GE/mL vs 1.000 (0.000–1.860) GE/mL (p=0.003); higher count of Peptostreptococcus spp. – 0.195 (0.000–1.560) GE/mL vs 0.000 (0.000-0.000) GE/mL (p=0.032); Eubacterium spp. – 1.355 (0.100–2.460) GE/mL vs 0.000 (0.000–1.560) GE/mL (p=0.040).
Conclusion. Endometrial dysbiosis in patients with a uterus scar defect after cesarean section due to the possible effects of microorganisms can be considered one of the leading causes of the formation of a uterus scar defect in the postoperative period.
作者简介
Anna Michelson
Urals Scientific Research Institute for Maternal and Child Care
Email: ann_lukach@list.ru
ORCID iD: 0000-0003-1709-6187
D. Sci. (Med.), Assoc. Prof.
俄罗斯联邦, YekaterinburgMargarita Telyakova
Urals Scientific Research Institute for Maternal and Child Care
编辑信件的主要联系方式.
Email: korovkari@gmail.com
ORCID iD: 0000-0001-7142-8547
Postgraduate Student
俄罗斯联邦, YekaterinburgMaria Lazukina
Urals Scientific Research Institute for Maternal and Child Care
Email: masha_balueva@mail.ru
ORCID iD: 0000-0002-0525-0856
Cand. Sci. (Med.)
俄罗斯联邦, YekaterinburgGuzel Chistyakova
Urals Scientific Research Institute for Maternal and Child Care
Email: guzel@niiomm.ru
ORCID iD: 0000-0002-0852-6766
D. Sci. (Med.), Prof.
俄罗斯联邦, YekaterinburgAleksandr Ustyuzhanin
Urals Scientific Research Institute for Maternal and Child Care
Email: ust103@yandex.ru
ORCID iD: 0000-0001-8521-7652
Cand. Sci. (Med.)
俄罗斯联邦, YekaterinburgAnatoly Varaksin
Institute of Industrial Ecology
Email: varaksinanatolij2@gmail.com
ORCID iD: 0000-0003-2689-3006
D. Sci. (Phys.-Math.), Prof.
俄罗斯联邦, YekaterinburgTatyana Maslakova
Institute of Industrial Ecology
Email: t9126141139@gmail.com
Cand. Sci. (Phys.-Math.)
俄罗斯联邦, YekaterinburgEkaterina Konstantinova
Institute of Industrial Ecology
Email: konstantinovaekateri@gmail.com
ORCID iD: 0000-0002-2260-744X
Cand. Sci. (Phys.-Math.)
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