Study of associations of polymorphic variants of lipid metabolism genes and angiogenesis with first ischemic stroke

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Abstract

Introduction. Ischemic stroke (IS) is a complex multifactorial disorder with heterogeneous congenital component. Polymorphic variants of genes of apolipoprotein В (APOB), apolipoprotein А-V (APOА-V), apolipoprotein С-IV (APOC-IV), apolipoprotein H (APOH), apolipoprotein D (APOD), angiopoietin-related protein 4 (ANGPT4) that regulate lipid metabolism and vascular regeneration, may be regarded as perspective ones. Although until recently they were not studied in complex, in a homogenous group from the perspective of possible association with first IS development. Aim. To study association between polymorphic variants of the following genes: АРОH, APOD, APOE, APOA5, APOC4, ANGPT4 and first IS development. Materials and methods. Frequencies of alleles and genotypes of single nucleotide polymorphic gene variants in 200 patients after first IS and in 115 people without stroke matched by sex, age and place of residence were studied. Blood was taken from all study participants in order to perform genotyping assay using protocol by J.Hixson and D.Vernier. Genomic deoxyribonucleic acid extraction from blood was performed using magnetic beads technology on an automated system for nucleic acid extraction Chemagen Prepito (ABBIS, Germany). For single nucleotide polymorphisms typing alleles identification analysis was performed using polymerase chain reaction with prepared TaqMan probes with Assey ID identification number (Applied Biosystems, USA). Amplification of polymorphic regions of the genes was performed with the use of 7500 Real-Time PCR System (Applied Biosystems, USA). Standard amplification conditions were used that were compatible with those defined by chemical agents supplier for each Assey ID. Genotypes nomenclature is given in accordance with international database db SNP (https://www.ncbi.nlm.nih.gov/SNP/). Results. Significant differences between groups in distribution of minor alleles and genotypes were acquired for АРОD (rs7659) and APOA5 (rs619054) polymorphisms. Conclusion. Significant association of single nucleotide АРОD (rs7659) and APOA-V (rs619054) genes polymorphisms with first IS development was found in study groups.

About the authors

Veronika N. Shishkova

Center for Speech Pathology and Neurorehabilitation; A.I.Yevdokimov Moscow State University of Medicine and Dentistry

Email: veronika-1306@mail.ru
Cand. Sci. (Med.), Senior Res. Officer

Alla Yu. Remennik

Center for Speech Pathology and Neurorehabilitation

Cand. Sci. (Med.)

Vera V. Valyaeva

Center for Speech Pathology and Neurorehabilitation

doctor-laboratory assistant

Tatiana V. Adasheva

A.I.Yevdokimov Moscow State University of Medicine and Dentistry

D. Sci. (Med.), Prof.

Ludmila V. Stakhovskaya

Pirogov Russian National Research Medical University

D. Sci. (Med.), Prof.

Ludmila A. Skipetrova

Center for Speech Pathology and Neurorehabilitation

Deputy Chief doctor

Roman A. Cheremin

Center for Speech Pathology and Neurorehabilitation

Cand. Sci. (Med.)

Viktor M. Shklovskii

Center for Speech Pathology and Neurorehabilitation

Full Prof.

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