Stereolithographic 3D Printing of Bioceramic Scaffolds of a Given Shape and Architecture for Bone Tissue Regeneration
- Authors: Putlyaev V.I.1, Yevdokimov P.V.1, Mamonov S.A.2, Zorin V.N.2, Klimashina E.S.1, Rodin I.A.1, Safronova T.V.1, Garshev A.V.1
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Affiliations:
- Moscow State University
- Experimental Production Workshops of the Federal Medical and Biological Agency of Russia
- Issue: Vol 10, No 5 (2019)
- Pages: 1101-1108
- Section: Materials for Ensuring Human Vital Activity and Environmental Protection
- URL: https://journals.rcsi.science/2075-1133/article/view/208079
- DOI: https://doi.org/10.1134/S2075113319050277
- ID: 208079
Cite item
Abstract
Abstract—A standard series of ceramic scaffolds for bone tissue regeneration with Kelvin architecture was developed, providing matrix water permeability not less than 900 Darcy and relative rigidity of matrices of not more than 0.2. The possibility of producing bioceramic scaffolds is shown using stereolithographic 3D printing of light-cured slurries containing a mixed calcium sodium phosphate Ca2.5Na(PO4)2 composition. A production technology of bioceramic scaffolds is developed and tested, including the development of photocurable slurries; 3D printing modes are also worked out, as well as conditions of heat treatment of printed models and sintering. The proposed methods of stereolithography formation followed by heat treatment of printed models make it possible to produce ceramic scaffolds with lateral resolution no worse than 50 μm and 50 μm layering. The dimensions of the bioceramic scaffold differ from the reference dimensions of the initial model by no more than 10%; the degree of macroporosity is not less than 70% and the pore size is 500 μm. It is shown that the obtained bioceramic scaffolds are compatible with the human fibroblast cell culture, are not cytotoxic, do not contain components inhibiting fibroblast adhesion, spreading, and proliferation, and can be used in tissue engineering.
About the authors
V. I. Putlyaev
Moscow State University
Author for correspondence.
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 119991
P. V. Yevdokimov
Moscow State University
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 119991
S. A. Mamonov
Experimental Production Workshops of the Federal Medical and Biological Agency of Russia
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 123182
V. N. Zorin
Experimental Production Workshops of the Federal Medical and Biological Agency of Russia
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 123182
E. S. Klimashina
Moscow State University
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 119991
I. A. Rodin
Moscow State University
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 119991
T. V. Safronova
Moscow State University
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 119991
A. V. Garshev
Moscow State University
Email: valery.putlayev@gmail.com
Russian Federation, Moscow, 119991