Email this article Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds
- Authors: Vasilyev A.V.1, Bukharova T.B.2, Kuznetsova V.S.1, Zagoskin Y.D.3, Minaeva S.A.4, Grigoriev T.E.3, Antonov E.N.4, Osidak E.O.5, Galitsyna E.V.2, Babichenko I.I.1, Domogatsky S.P.6, Popov V.K.4, Chvalun S.N.3, Goldshtein D.V.2, Kulakov A.A.1
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Affiliations:
- Central Research Institute of Dentistry and Maxillofacial Surgery
- Research Centre for Medical Genetics
- National Research Center Kurchatov Institute
- Federal Scientific Research Centre Crystallography and Photonics, Russian Academy of Sciences
- Imtek Company Ltd.
- Cardiology Research and Production Center, Ministry of Health of the Russian Federation
- Issue: Vol 10, No 5 (2019)
- Pages: 1093-1100
- Section: Materials for Ensuring Human Vital Activity and Environmental Protection
- URL: https://journals.rcsi.science/2075-1133/article/view/208077
- DOI: https://doi.org/10.1134/S2075113319050332
- ID: 208077
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Abstract
The purpose of this study was to evaluate the release kinetics of impregnated recombinant human bone morphogenetic protein-2 (rhBMP-2) from different engineered scaffolds. Poly(lactide-co-glycolide) (PLG) matrices prepared by supercritical fluid technologies (SCFT) showed the highest biocompatibility and long-term release of rhBMP-2. There was an even release of rhBMP-2 from them for 11 days. The subsequent use of laser sintering allowed delaying the peak of the protein release for a period of 13 to 15 days. The average loss of rhBMP-2 using SCFT did not exceed 20%. The maximum release of rhBMP-2 from a collagen-fibronectin hydrogel was in the period from 4 to 6 days. But 47 ± 12% rhBMP-2 loss was shown. Highly porous polylactide-based scaffolds obtained by freeze-drying were inferior to other scaffolds in their ability to release rhBMP-2 for a prolonged period. The hydrogel and chitosan-based granules showed high cytotoxicity and a short period of protein release.
About the authors
A. V. Vasilyev
Central Research Institute of Dentistry and Maxillofacial Surgery
Author for correspondence.
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 119021
T. B. Bukharova
Research Centre for Medical Genetics
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 115522
V. S. Kuznetsova
Central Research Institute of Dentistry and Maxillofacial Surgery
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 119021
Yu. D. Zagoskin
National Research Center Kurchatov Institute
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 123182
S. A. Minaeva
Federal Scientific Research Centre Crystallography and Photonics, Russian Academy of Sciences
Email: vav-stom@yandex.ru
Russian Federation, Moscow, Troitsk, 142190
T. E. Grigoriev
National Research Center Kurchatov Institute
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 123182
E. N. Antonov
Federal Scientific Research Centre Crystallography and Photonics, Russian Academy of Sciences
Email: vav-stom@yandex.ru
Russian Federation, Moscow, Troitsk, 142190
E. O. Osidak
Imtek Company Ltd.
Email: vav-stom@yandex.ru
Russian Federation, Moscow
E. V. Galitsyna
Research Centre for Medical Genetics
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 115522
I. I. Babichenko
Central Research Institute of Dentistry and Maxillofacial Surgery
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 119021
S. P. Domogatsky
Cardiology Research and Production Center, Ministry of Health of the Russian Federation
Email: vav-stom@yandex.ru
Russian Federation, Moscow
V. K. Popov
Federal Scientific Research Centre Crystallography and Photonics, Russian Academy of Sciences
Email: vav-stom@yandex.ru
Russian Federation, Moscow, Troitsk, 142190
S. N. Chvalun
National Research Center Kurchatov Institute
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 123182
D. V. Goldshtein
Research Centre for Medical Genetics
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 115522
A. A. Kulakov
Central Research Institute of Dentistry and Maxillofacial Surgery
Email: vav-stom@yandex.ru
Russian Federation, Moscow, 119021
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