Effects of Synthesis Method and Stabilizing Agent Trehalose on Functional Properties of Protein Composites with Colloidal Silica

Abstract

Protein-silica composites are a promising platform for the development of new dosage of protein drugs. The sol-gel method was used to synthesize bovine serum albumin composites with colloidal silica in the presence and absence of trehalose as a protein structure stabilizer. The structural state of the protein in the composites and after release from them and the kinetics and mechanisms of in vitro release were studied. The functional properties of the sol-gel composites were compared with similar composites obtained by the adsorption method. The strong effect of the synthesis method and the influence of trehalose on the structure of the protein and the kinetic parameters and mechanisms of its release were shown. On the basis of a comparative analysis, it was concluded that sol-gel composites have a number of advantages over adsorption-derived composites in terms of their functioning as delivery systems of protein drugs.