Biocatalysts Based on Bacterial Cells with Amidase Activity for the Synthesis of Acrylic Acid from Acrylamide


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详细

The biocatalytic synthesis of acrylic acid from acrylamide by the Rhodococcus erythropolis 4-1 and Alcaligenes faecalis 2 strains with amidase activity is studied. The optimum pH values are 6–7 for R. erythropolis 4-1 and 7–7.5 for A. faecalis 2, while the optimum temperature is 20–50°С for both strains. The optimum acrylamide concentration is 150 mM for R. erythropolis 4-1 and 250 mM for A. faecalis 2. The synthesis of acrylic acid with fractional additions of a substrate catalyzed by the biomass of A. faecalis 2 is more effective than using R. erythropolis 4-1. The biocatalyst is best stored at −20°C. The amidase activity of A. faecalis 2 cells immobilized on chitosan activated with glutaraldehyde and non-activated chitosan did not decline during the storage of either wet or dried granules.

作者简介

Yu. Maksimova

Institute of Ecology and the Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm State National Research University

编辑信件的主要联系方式.
Email: yul_max@mail.ru
俄罗斯联邦, Perm, 614081; Perm, 614990

M. Yakimova

Perm National Research Polytechnic University

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Email: yakimova.maryas@yandex.ru
俄罗斯联邦, Perm, 614990

A. Maksimov

Institute of Ecology and the Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences; Perm National Research Polytechnic University; Perm State National Research University

编辑信件的主要联系方式.
Email: almaks1@mail.ru
俄罗斯联邦, Perm, 614081; Perm, 614990; Perm, 614990

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