Email this article Using an Inducible Promoter of the Glucoamylase Gene to Construct New Multienzyme Complexes from Penicillium verruculosum
- Authors: Sinitsyn A.P.1,2, Volkov P.V.3, Rubtsova E.A.3, Shashakov I.A.3, Rozhkova A.M.3, Sinitsyna O.A.1, Kondrat’eva E.G.3, Zorov I.N.1,3, Satrudinov A.D.3, Merzlov D.A.1, Matys V.Y.4
-
Affiliations:
- Moscow State University
- National Research Institute of Food Biotechnology, Division of the Federal Research Center for Food and Biotechnology
- Federal Research Center of Biotechnology
- Institute of Biochemistry and Physiology of Microorganisms
- Issue: Vol 10, No 1 (2018)
- Pages: 75-82
- Section: Biocatalysis
- URL: https://journals.rcsi.science/2070-0504/article/view/202758
- DOI: https://doi.org/10.1134/S2070050418010105
- ID: 202758
Cite item
Open Access
Access granted
Subscription Access
Abstract
New recombinant strains of Penicillium verruculosum are created using a new plasmid construct based on an inducible promoter of glucoamylase gene (gla1) that secretes heterologous xylanase E (XylE) from P. canescens. New biocatalysts are produced that contain cellulolytic enzyme preparations (EPs) enriched with XylE. The amount of XylE in the recombinant EPs varies in the optimum range of 11–24% of the total protein while generally preserving the P. verruculosum cellulose complex. The hydrolytic activity of the new EPs with respect to polymeric plant-derived substrates exceeds that of EPs produced using other expression systems and commercial preparations. The new EP glaX-17 surpasses in particular a control EP based on the recipient strain by 13% in the efficiency of hydrolyzing aspen wood and is 20% more efficient than the commercial EP Accelerase Duet. The new EP glaX-17 displays 25% greater efficiency (35–43%) during the hydrolysis of wheat bran than the commercial EP Accelease Duet. The effectiveness is demonstrated of using the new gla1 promoter for the production of EPs (biocatalysts) while preserving the balanced cellulose complex of the strain and optimum yield of heterologous XylE required for the deep hydrolysis of xylan-containing plant biomass.
About the authors
A. P. Sinitsyn
Moscow State University; National Research Institute of Food Biotechnology, Division of the Federal Research Center for Food and Biotechnology
Author for correspondence.
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119991; Moscow, 111033
P. V. Volkov
Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119071
E. A. Rubtsova
Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119071
I. A. Shashakov
Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119071
A. M. Rozhkova
Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119071
O. A. Sinitsyna
Moscow State University
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119991
E. G. Kondrat’eva
Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119071
I. N. Zorov
Moscow State University; Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119991; Moscow, 119071
A. D. Satrudinov
Federal Research Center of Biotechnology
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119071
D. A. Merzlov
Moscow State University
Email: apsinitsyn@gmail.com
Russian Federation, Moscow, 119991
V. Yu. Matys
Institute of Biochemistry and Physiology of Microorganisms
Email: apsinitsyn@gmail.com
Russian Federation, Pushchino, 142290
Supplementary files
