Optimizing the composition of cellulase enzyme complex from Penicillium verruculosum: Enhancing hydrolytic capabilities via genetic engineering
- Авторлар: Sinitsyn A.P.1,2, Korotkova O.G.2, Sinitsyna O.A.1, Rozhkova A.M.1,2, Dotsenko G.S.3, Proskurina O.V.2, Osipov D.O.2, Kondrat’eva E.G.2, Chekushina A.V.2
- 
							Мекемелер: 
							- Faculty of Chemistry
- Federal Research Center for the Fundamental Basics of Biotechnology
- Technical University of Denmark
 
- Шығарылым: Том 8, № 1 (2016)
- Беттер: 101-106
- Бөлім: Biocatalysis
- URL: https://journals.rcsi.science/2070-0504/article/view/202163
- DOI: https://doi.org/10.1134/S2070050416010128
- ID: 202163
Дәйексөз келтіру
Аннотация
Modern technologies for the enzyme hydrolysis of cellulose-containing raw materials allow the production of sugars from which alcohols (biofuel), organic and amino acids, biopolymers, feed additives, and other value-added products can be obtained via microbiological conversion. Three types of cellulolytic enzymes are required for the bioconversion of cellulose containing materials: endoglucanase, cellobiohydrolase, and ß-glucosidase. The prospects for improving the hydrolytic capabilities of the enzyme complex secreted from Penicillium verruculosum are investigated in this work by means of genetic engineering to add different combinations and ratios of homologous and heterologous cellulases: endoglucanase IV (EGIV) of Trichoderma reesei, endoglucanase II (EGII), and cellobiohydrolase I (CBHI) of P. verruculosum, along with ß-glucosidase (ß-GLU) of Aspergillus niger. The optimum ratio of components is determined and the catalytic activity of enzymatic complexes is increased by as much as 100%.
Авторлар туралы
A. Sinitsyn
Faculty of Chemistry; Federal Research Center for the Fundamental Basics of Biotechnology
							Хат алмасуға жауапты Автор.
							Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 119991; Moscow, 117071						
O. Korotkova
Federal Research Center for the Fundamental Basics of Biotechnology
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 117071						
O. Sinitsyna
Faculty of Chemistry
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 119991						
A. Rozhkova
Faculty of Chemistry; Federal Research Center for the Fundamental Basics of Biotechnology
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 119991; Moscow, 117071						
G. Dotsenko
Technical University of Denmark
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Дания, 							Kongens Lyngby, 2800						
O. Proskurina
Federal Research Center for the Fundamental Basics of Biotechnology
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 117071						
D. Osipov
Federal Research Center for the Fundamental Basics of Biotechnology
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 117071						
E. Kondrat’eva
Federal Research Center for the Fundamental Basics of Biotechnology
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 117071						
A. Chekushina
Federal Research Center for the Fundamental Basics of Biotechnology
														Email: apsinitsyn@gmail.com
				                					                																			                												                	Ресей, 							Moscow, 117071						
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