Purification of Xenogeneic Bone Matrix by Extraction with Supercritical Carbon Dioxide and Evaluation of the Obtained Material

Abstract

An environmentally friendly method of osteoplastic material production is proposed for reconstructive surgery needs based on treatment of xenogeneic bone matrix with supercritical carbon dioxide. The method provides the best extraction of lipids and fatlike substances at minimum cost of extracting agent and processing time; it allows the significant reduction of costs and facilitates the manufacture of surgical implants. The advantages of the obtained material in comparison with the known commercial analogue are demonstrated by in vitro experiments on the cellular model.