Email this article Representation Analysis of miRNA in Urine Microvesicles and Cell-Free Urine in Prostate Diseases
- Authors: Zaporozhchenko I.A.1,2, Bryzgunova O.E.1,2, Lekchnov E.A.1,2, Osipov I.D.3, Zaripov M.M.4, Yurchenko Y.B.1, Yarmoschuk S.V.2, Pashkovskaya O.A.2, Rykova E.Y.1,2, Zheravin A.A.2, Laktionov P.P.1,2
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Affiliations:
- Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
- Meshalkin Siberian Federal Biomedical Research Center
- Novosibirsk State University
- Siberian District Medical Center
- Issue: Vol 12, No 2 (2018)
- Pages: 156-163
- Section: Article
- URL: https://journals.rcsi.science/1990-7508/article/view/198203
- DOI: https://doi.org/10.1134/S1990750818020142
- ID: 198203
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Abstract
Urine of prostate cancer patients contains tumor-specific biopolymers, including protein- and microvesicles-associated miRNAs that can potentially be used as oncomarkers. Previously we have characterized urinary extracellular vesicles and demonstrated diagnostic potential of their miRNAs. In this study, we have performed a comparative analysis of 84 miRNA in paired samples of urine microvesicles and urine supernatant from healthy men, patients with benign prostate hyperplasia, and prostate cancer patients using miRCURY LNA miRNA qPCR Panels. In all groups of patients, miRNA subsets characterized by different distribution between the urinary fractions have been found. In this context, two groups of miRNAs have been identified, which are involved in several signaling pathways including those associated with prostate cancer development.
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About the authors
I. A. Zaporozhchenko
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences; Meshalkin Siberian Federal Biomedical Research Center
Author for correspondence.
Email: ivanzap@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090; Novosibirsk
O. E. Bryzgunova
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences; Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090; Novosibirsk
E. A. Lekchnov
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences; Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090; Novosibirsk
I. D. Osipov
Novosibirsk State University
Email: ivanzap@niboch.nsc.ru
Russian Federation, Novosibirsk
M. M. Zaripov
Siberian District Medical Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, Novosibirsk
Yu. B. Yurchenko
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences
Email: ivanzap@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090
S. V. Yarmoschuk
Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, Novosibirsk
O. A. Pashkovskaya
Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, Novosibirsk
E. Yu. Rykova
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences; Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090; Novosibirsk
A. A. Zheravin
Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, Novosibirsk
P. P. Laktionov
Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences; Meshalkin Siberian Federal Biomedical Research Center
Email: ivanzap@niboch.nsc.ru
Russian Federation, pr. Akad. Lavrentieva 8, Novosibirsk, 630090; Novosibirsk
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