The Influence of Enzymatic Removal of Chlorpyrifos from Feed Grain Mixes on Biochemical Parameters of Rat Blood

Abstract

Organophosphorus pesticides (OP) are used to protect crops from pests. Treatment of plants and animals with pesticides can be done during their growth or creation of conditions required for the long-shelf life of the agricultural products. Currently, many remedies exist for prevention and removal of intoxication consequences developed in living organisms exposed to OPs. The development of biologics for degradation of OPs and biotechnologies for their application in agriculture still represents an important task. New biologics based on stabilized forms of such enzyme as hexahistidine-tagged organophosphorus hydrolase (His₆-OPH) have been in the form of nano-sized particles for OPs detoxification. They represent enzyme-polyelectrolyte complexes (EPC) obtained by mixing solutions of His₆-OPH and polyanion under certain conditions. The main purpose of this work was to evaluate the usage efficiency of EPC based on His₆-OPH and polyglutamic acid for OPs detoxification by analyzing biochemical blood parameters of rats fed with a grain mix containing chlorpyrifos. The experiment was conducted using female Sprague-Dowly albino rats. Treatment of the feeding grain mix initially containing chlopyrifos (48 mg/kg of the mix) with EPC based on His₆-OPH (1000 U/kg of the mix) for 24 h was the most effective. The results showed that acetyl cholinesterase activity in blood of rats from the group consuming food after the enzymatic removal of chlorpyrifos, was comparable to acetyl cholinesterase activity in blood of rats consuming pure food.