Methods for Determination of Functional Activity of Cytochrome P450 Isoenzymes


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Abstract

The review deals with modern methods and technologies for determination of functional activity cytochrome P450 isoenzymes; these include absorbance and fluorescent spectroscopy, electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR), Raman, Mössbauer, and X-ray spectroscopy, surface plasmon resonance (SPR), atomic force microscopy (AFM). Methods of molecular genetic analysis have been considered in the context of personalized medicine. Using the methods of chromatography mass spectrometry it is possible to analyze reaction products formed in reactions catalyzed by cytochromes P450. Special attention is paid to modern electrochemical systems based on cytochrome P450 isoenzymes, their applicability for analysis of their catalytic activity, their use in practice and further development perspectives for experimental pharmacology, biotechnology and translational medicine.

About the authors

A. V. Kuzikov

Institute of Biomedical Chemistry (IBMC); Pirogov Russian National Research Medical University (RNRMU)

Email: v_shumyantseva@mail.ru
Russian Federation, ul. Pogodinskaya 10, Moscow, 119121; Moscow

R. A. Masamrekh

Institute of Biomedical Chemistry (IBMC); Pirogov Russian National Research Medical University (RNRMU)

Email: v_shumyantseva@mail.ru
Russian Federation, ul. Pogodinskaya 10, Moscow, 119121; Moscow

A. I. Archakov

Institute of Biomedical Chemistry (IBMC); Pirogov Russian National Research Medical University (RNRMU)

Email: v_shumyantseva@mail.ru
Russian Federation, ul. Pogodinskaya 10, Moscow, 119121; Moscow

V. V. Shumyantseva

Institute of Biomedical Chemistry (IBMC); Pirogov Russian National Research Medical University (RNRMU)

Author for correspondence.
Email: v_shumyantseva@mail.ru
Russian Federation, ul. Pogodinskaya 10, Moscow, 119121; Moscow

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