Possibility of Application of Extracellular Protease of the Micromycetе Aspergillus ochraceus VKM F-4104D for Determination of the Protein C Content in Human Blood Plasma

Abstract

The protein C activator activity, determined in normal plasma by using A. ochraceus protease is comparable with the activity of a commercial protease analogue from the South American copperhead venom (Protac®). As in the case of Protac®, the A. ochraceus protease can be used for protein C determination in plasma with its reduced content. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may be a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.