Specificity of Isatin Interaction with Cytochromes P450
- Authors: Ershov P.V.1, Mezentsev Y.V.1, Yablokov E.O.1, Kaluzhskiy L.A.1, Florinskaya A.V.1, Svirid A.V.2, Gilep A.A.2, Usanov S.A.2, Medvedev A.E.1, Ivanov A.S.1
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Affiliations:
- Institute of Biomedical Chemistry
- Institute of Bioorganic Chemistry
- Issue: Vol 12, No 2 (2018)
- Pages: 130-135
- Section: Article
- URL: https://journals.rcsi.science/1990-7508/article/view/198162
- DOI: https://doi.org/10.1134/S1990750818020026
- ID: 198162
Cite item
Abstract
Cytochrome P450-dependent monooxygenase systems exist basically in all living organisms, where they perform various important functions. The coordinated functioning of these systems involves many proteins participating in different protein-protein interactions (PPI). Previously, we have found that the endogenous non-peptide bioregulator isatin (indoledione-2,3), synthesized from indole by means of certain cytochromes P450 (e.g. P450 2E1, P450 2C19, P450 2A6) regulates affinity of some PPI. In this study, an attempt has been undertaken to register a direct interaction of isatin with a set of different proteins related to the functioning of cytochrome P450-dependent monooxygenase systems: five isoforms of cytochromes P450, two isoforms of cytochrome b5, cytochrome P450 reductase, adrenodoxin, adrenodoxin reductase and ferrochelatase. The study has shown high affinity specific binding of isatin only to cytochromes P450 (the equilibrium dissociation constant (Kd) is about 10–8 M).
About the authors
P. V. Ershov
Institute of Biomedical Chemistry
Author for correspondence.
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
Yu. V. Mezentsev
Institute of Biomedical Chemistry
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
E. O. Yablokov
Institute of Biomedical Chemistry
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
L. A. Kaluzhskiy
Institute of Biomedical Chemistry
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
A. V. Florinskaya
Institute of Biomedical Chemistry
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
A. V. Svirid
Institute of Bioorganic Chemistry
Email: pavel79@inbox.ru
Belarus, ul. Akad. Kuprevicha 5/2, Minsk, 220141
A. A. Gilep
Institute of Bioorganic Chemistry
Email: pavel79@inbox.ru
Belarus, ul. Akad. Kuprevicha 5/2, Minsk, 220141
S. A. Usanov
Institute of Bioorganic Chemistry
Email: pavel79@inbox.ru
Belarus, ul. Akad. Kuprevicha 5/2, Minsk, 220141
A. E. Medvedev
Institute of Biomedical Chemistry
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
A. S. Ivanov
Institute of Biomedical Chemistry
Email: pavel79@inbox.ru
Russian Federation, Pogodinskaya ul. 10, Moscow, 119121
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