Bacterial TEM-Type Serine Beta-Lactamases: Structure and Analysis of Mutations
- 作者: Grigorenko V.G.1, Rubtsova M.Y.1, Uporov I.V.1, Ishtubaev I.V.1, Andreeva I.P.1, Shcherbinin D.S.1,2, Veselovsky A.V.3, Egorov A.M.1
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隶属关系:
- Chemical Department of Moscow State University
- Center for Data-Intensive Biomedicine and Biotechnology
- Institute of Physiologically Active Compounds
- 期: 卷 12, 编号 2 (2018)
- 页面: 87-95
- 栏目: Article
- URL: https://journals.rcsi.science/1990-7508/article/view/198138
- DOI: https://doi.org/10.1134/S1990750818020038
- ID: 198138
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详细
Beta-lactamases (EC 3.5.2.6) represent a superfamily containing more than 2000 members: it includes genetically and functionally different bacterial enzymes capable to degrade the beta-lactam antibiotics. Beta-lactamases of molecular class A with serine residue in the active center are the most common ones. In the context of studies of the mechanisms underlying of evolution of the resistance, TEM type beta-lactamases are of particular interest due to their broad polymorphism. To date, more than 200 sequences of TEM type beta-lactamases have been described and more than 60 structures of different mutant forms of these enzymes have been presented in the Protein Data Bank. We have considered here the main structural features of the enzymes of this type with particular attention to the analysis of key mutations determining drug resistance and the secondary mutations, their location relative to the active center and the surface of the protein globule. We have developed a BlaSIDB database (www.blasidb.org) which is an open information resource combining available data on 3D structures, amino acid sequences and nomenclature of the TEM type beta-lactamases.
作者简介
V. Grigorenko
Chemical Department of Moscow State University
编辑信件的主要联系方式.
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991
M. Rubtsova
Chemical Department of Moscow State University
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991
I. Uporov
Chemical Department of Moscow State University
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991
I. Ishtubaev
Chemical Department of Moscow State University
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991
I. Andreeva
Chemical Department of Moscow State University
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991
D. Shcherbinin
Chemical Department of Moscow State University; Center for Data-Intensive Biomedicine and Biotechnology
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991; Moscow
A. Veselovsky
Institute of Physiologically Active Compounds
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow region, Chernogolovka
A. Egorov
Chemical Department of Moscow State University
Email: vitaly.grigorenko@gmail.com
俄罗斯联邦, Moscow, 119991
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