Morphofunctional changes of dendritic cells induced by sulfated polysaccharides of brown algae

Abstract

The effect of structurally different sulfated polysaccharides from brown algae Fucus evanescens, Saccharina cichorioides, and Saccharina japonica on the morphofunctional changes of dendritic cells has been studied using flow cytometry and phase-contrast microscopy. It has been found that the dendritic cells are characterized by larger sizes, vacuolated cytoplasm, eccentrically located nucleus, the presence of numerous cytoplasmic pseudopodia of various forms; they do express surface markers, thus providing evidence for their maturation (CD83, CD11c, HLA-DR, CD86). Increased production of immunoregulatory (IL-12) and proinflammatory cytokines (TNF-α, IL-6) by dendritic cells polarizes the development of the Th-1 type immune response.