The role of neutrophil myeloperoxidase in the development of inflammation induced by thermal skin burns
- Authors: Mikhalchik E.V.1, Budkevich L.I.2, Piterskaya Y.A.2, Penkov L.Y.2, Astamirova T.S.2, Smolina N.V.1, Vakhrusheva T.V.1, Panasenko O.M.1
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Affiliations:
- Federal Research and Clinical Center of Physical-Chemical Medicine
- Speransky Pediatric Clinical Hospital no. 9
- Issue: Vol 11, No 1 (2017)
- Pages: 72-75
- Section: Article
- URL: https://journals.rcsi.science/1990-7508/article/view/197643
- DOI: https://doi.org/10.1134/S1990750817010073
- ID: 197643
Cite item
Abstract
Luminol-dependent chemiluminescence (CL) of blood neutrophils stimulated with phorbol-12- myristate-13-acetate (PMA) and myeloperoxidase (MPO) activity of neutrophils and plasma have been investigated in children (n = 16) during the early period (1−7 days) after thermal skin burns exceeding 20% of total body surface. The CL level of stimulated neutrophils was higher in burn patients than in healthy children of the reference group (p lt; 0.01). Increased neutrophil MPO activity was found in 40% of patients, while increased plasma MPO activity was detected in 57% of patients. The albumin fraction isolated from plasma of burn patients increased the PMA-stimulated CL response of blood from healthy donors. These results suggest that the acute inflammatory response to the thermal burn causes neutrophil activation and MPO release into plasma. MPO-mediated modification of plasma proteins, particularly albumin, may stimulate neutrophil activation and provoke further inflammatory response of the body to the thermal injury.
Keywords
About the authors
E. V. Mikhalchik
Federal Research and Clinical Center of Physical-Chemical Medicine
Author for correspondence.
Email: lemik2007@yandex.ru
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119435
L. I. Budkevich
Speransky Pediatric Clinical Hospital no. 9
Email: lemik2007@yandex.ru
Russian Federation, Moscow
Yu. A. Piterskaya
Speransky Pediatric Clinical Hospital no. 9
Email: lemik2007@yandex.ru
Russian Federation, Moscow
L. Yu. Penkov
Speransky Pediatric Clinical Hospital no. 9
Email: lemik2007@yandex.ru
Russian Federation, Moscow
T. S. Astamirova
Speransky Pediatric Clinical Hospital no. 9
Email: lemik2007@yandex.ru
Russian Federation, Moscow
N. V. Smolina
Federal Research and Clinical Center of Physical-Chemical Medicine
Email: lemik2007@yandex.ru
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119435
T. V. Vakhrusheva
Federal Research and Clinical Center of Physical-Chemical Medicine
Email: lemik2007@yandex.ru
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119435
O. M. Panasenko
Federal Research and Clinical Center of Physical-Chemical Medicine
Email: lemik2007@yandex.ru
Russian Federation, ul. Malaya Pirogovskaya 1a, Moscow, 119435