Differences between integrin α5β1 and EGRF receptor in signal pathways controlling proliferation and apoptosis of MCF-7/Dox human breast carcinoma cells


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In MCF-7/Dox human breast carcinoma cells, down-regulation of integrin α5β1 and inhibition of epidermal growth factor receptor (EGFR) markedly reduced cell proliferation. Cell cycle analysis showed that α5β1 down-regulation resulted in cycle arrest at the S-phase, followed by a significant increase in the population of apoptotic cells (subG1 population). Inhibition of EGFR activity also caused cell cycle arrest at the S-phase but without any increase in the subG1 population. Down-regulation of α5β1 and EGFR inhibition resulted in a significant decrease of cell content of the active (phosphorylated) forms of FAK and Erk protein kinases. The data obtained suggest that α5β1 integrin is implicated in cell growth control via inhibition of apoptotic cell death and through EGFR activation.

Sobre autores

N. Kozlova

Institute of Biomedical Chemistry

Email: 1938berman@gmail.com
Rússia, ul. Pogodinskaya 10, Moscow, 119121

G. Morozevich

Institute of Biomedical Chemistry

Email: 1938berman@gmail.com
Rússia, ul. Pogodinskaya 10, Moscow, 119121

N. Ushakova

Institute of Biomedical Chemistry

Email: 1938berman@gmail.com
Rússia, ul. Pogodinskaya 10, Moscow, 119121

N. Gevorkian

Institute of Biomedical Chemistry

Email: 1938berman@gmail.com
Rússia, ul. Pogodinskaya 10, Moscow, 119121

A. Berman

Institute of Biomedical Chemistry

Autor responsável pela correspondência
Email: 1938berman@gmail.com
Rússia, ul. Pogodinskaya 10, Moscow, 119121

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