Lipid peroxidation in cardiac mitochondrial fraction of rats exposed to different supplementation with polyunsaturated fatty acids

Abstract

The effect of diet supplementation with polyunsaturated fatty acids (PUFAs) used at different ratios of ω-6/ω-3 on the content of primary (diene conjugates, DC; triene conjugates, TC), secondary (ketodienes, CD; coupled trienes, CT; TBA-active products) and terminal (Schiff bases) lipid peroxidation products (LPO) and generation of superoxide anion-radical was studied in rat cardiac mitochondrial fraction. The cardiac mitochondrial fraction of rats kept on a diet with a high content of ω-6 and ω-3 PUFAs for eight weeks was characterized by increased content the primary, secondary and final LPO and a higher rate of superoxide radical formation. In the case of diet supplementation with ω-6 and ω-3 PUFAs used at the ratio of 4: 1, the leading factor determining LPO intensity in the cardiac mitochondrial fraction is a species PUFA composition rather than the degree of saturation.