Participation of Endocytosis in Sodium Ion Uptake by the Cells of Arabidopsis thaliana (L.) Heynh in the Suspension Culture
- 作者: Orlova Y.1, Majorova O.1, Khalilova L.1, Voronkov A.1, Fomenkov A.1,2, Nosov A.1,2, Popova L.1, Balnokin Y.1,3
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隶属关系:
- Timiryasev Plant Physiology Institute, Russian Academy of Sciences
- Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
- Faculty of Biology, Moscow Lomonosov State University
- 期: 卷 12, 编号 4 (2018)
- 页面: 382-389
- 栏目: Articles
- URL: https://journals.rcsi.science/1990-7478/article/view/213330
- DOI: https://doi.org/10.1134/S1990747818050045
- ID: 213330
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详细
The involvement of endocytosis in the Na+ ion uptake from the external medium by the cells of suspension culture derived from A. thaliana (Col-0) leaves was investigated. Na+ ion uptake by endocytic structures occurred following the addition of NaCl at the final concentration of 100 mM to the incubation medium. The presence of Na+ in membranous structures was recorded using fluorescence microscopy by colocalization of FM4-64, a marker of endocytosis structures, and Asante NaTRIUM Green-2 TMA+ salt (ANG-2 TMA), a membrane impermeable probe for sodium ions, that enabled the detection of Na+ absorbed by the cells via endocytosis but not through ion channels or transporters of the plasma membrane. Following a 1.5-h incubation of the cells in the presence of NaCl, FM4-64 and ANG-2 TMA, fluorescence of the probes was colocalized in structures with sizes ranging from 800 to 3000 nm. It was shown by electron microscopy that NaCl added to the cell incubation medium stimulated vesiculation and vacuolization of the cytoplasm, formation of plasma membrane invaginations, as well as fusion of microvacuoles with each other. The size of the structures, in which the colocalization of the two probes was detected by fluorescent microscopy, matched the size of the microvacuoles revealed by the electron microscopy. The obtained results indicate the capture of sodium ions contained in the apoplast by endocytosis invaginations, their subsequent internalization by the cells, and transfer into microvacuoles.
作者简介
Y. Orlova
Timiryasev Plant Physiology Institute, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276
O. Majorova
Timiryasev Plant Physiology Institute, Russian Academy of Sciences
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276
L. Khalilova
Timiryasev Plant Physiology Institute, Russian Academy of Sciences
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276
A. Voronkov
Timiryasev Plant Physiology Institute, Russian Academy of Sciences
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276
A. Fomenkov
Timiryasev Plant Physiology Institute, Russian Academy of Sciences; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276; Novosibirsk, 630090
A. Nosov
Timiryasev Plant Physiology Institute, Russian Academy of Sciences; Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276; Novosibirsk, 630090
L. Popova
Timiryasev Plant Physiology Institute, Russian Academy of Sciences
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276
Y. Balnokin
Timiryasev Plant Physiology Institute, Russian Academy of Sciences; Faculty of Biology, Moscow Lomonosov State University
Email: orl-jul@mail.ru
俄罗斯联邦, Moscow, 127276; Moscow, 119992